Any help about isolating fibroblasts for primary cell culture, from human skin biopsy?

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Volunteer for Article Reviewer with Education areas

Need Volunteer for Article Reviewer with Education areas of expertise and have Google Scholar / Scopus ID/ or Orcid ID/ publons ID Profile with several collections of the results of writing the...

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What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

How can we simulate an hyperelastic-viscoelastic behavor with Ansys APDL?

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28 February 2021 552 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

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23 February 2021 7,326 5 View

Alginate and silk fibroin hydrogels toxic to cells?

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FDC and FRC cell populations in mouse spleen?

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17 February 2021 3,506 4 View

What to do with absence of fluorescence label in Fucci2a mouse-derived fibroblast culture?

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Can ImageJ software be used to quantify collagen from Masson's-Trichrome stained images?

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Non motile black dots in animal cell culture, tightly adhered to the cells along the cell periphery. Is it a contamination? How to get rid of them?

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18 January 2021 5,151 1 View

Does use of hand sanitizer negatively impact the skin microbiome?

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16 January 2021 6,923 11 View

Aleksandra Czumaj

In the case of dermal fibroblast collagenase solution and dispase solution work pretty well. The most crucial step is tissue sampling and transport. Tissue should be processed as soon as possible after surgery, till then keep the sample at 4 °C

Ron Hrstka

Alternatively you can cut the explants into small pieces and put a cover slip over them to hold them down on the bottom of the plate. Remove the coverslip after about a week.