We need biochemist partners to perform MAO-B inhibitor or other enzyme inhibition activities on oue newly synthesized propargylamines.
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RBCs have auto-fluorescence, I want to detect that auto-fluorescence in paraffin embedded tissue samples. Any suggestions?
08 February 2021 9,357 1 View
Hello everyone I did the RT PCR by using cyber green as a fluorescent dye however when I run the RT PCR of my two strains of Saccharomyces cerevisiae (duplicate) then I got the result in which the...
21 November 2020 2,076 3 View
Need to write an extended version of my work which is selected to publish in a journal.
28 May 2020 2,607 4 View
Can anybody help with interpretation of FTIR Graphs to read peaks of graphs for fruit bars made from fruits, herb and cereal?
18 May 2020 6,159 2 View
Suppose I need to calculate A =(B/C), where B= 1.00+- 0.33 and C= 1.50+- 0.43 How do I calculate the error of A? as in, A=0.66+- ? Any idea if I can do this in excel or any other...
13 April 2020 7,667 3 View
after the interaction of drug the enhancement in the SPR band was observed the intewraction was proved by FTIR and AFM the AFM resiults shows aggregation of NPs the of NPS increases from 40nm to...
10 February 2020 9,355 3 View
its colorimetric detection after interacting a analye with nanoparticles color rapidly change from yellow to orange. after 15 min of interaction take of a absorption spectra only quenching...
04 November 2019 7,712 1 View
I want to use E. faecalis JH22 as competent cells to express my protein using anhydrotetracycline (ATc) to induce protein expression i need some standard protocol if someone has use it before.
26 July 2019 1,979 2 View
I am trying to make chitosan film (of various concentrations)on glass substrate by simple casting method from solution in acetic acid. After the solution dries, film sticks to glass so firmly that...
11 June 2019 7,504 3 View
Hello Everyone, Need quick help! I have 5 different rates for recurrence from 5 studies with different followup. How do I combine them to find one probability to be used in cost-effectiveness...
05 November 2017 4,107 7 View
My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...
01 March 2021 6,474 3 View
Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...
23 February 2021 7,429 1 View
I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...
21 February 2021 5,379 2 View
I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...
21 February 2021 6,260 3 View
We want to use phenol pH graphic to measure the pH of mediums before and after inhibitor application to cells. We have phenol absorbence values at 460, 560 and 720 nm wavelength. When we measure...
20 February 2021 7,700 3 View
I'm planning to use ST078925 OGT inhibitor from TimTec (OGT-O-linked N-acetylglucosamine (GlcNAc) transferase) for female rats.
16 February 2021 7,850 2 View
Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...
14 February 2021 2,610 1 View
Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...
12 February 2021 1,386 3 View
Hello! I want to determine the activity of my in house Reverse Transcriptase enzyme. For this, I have used Thermo's Enzcheck Reverse Transcriptase assay kit. I used commercial Thermo RT enzyme to...
08 February 2021 7,285 1 View
We aim to use MK-2206 to inhibit AKT in mice. However, a large body of studies suggests that it should be administered orally. I was wondering if somebody could tell me that is it toxic for the...
07 February 2021 6,340 1 View