Any good workshops or conferences on protein-protein interactions?

More Gaurav Chhetri's questions See All

No Fluorescence signals in GFP based imaging and expression?

We have prepared a construct using GFP sequences in middle. Although sequencing analysis confirmed the positive clone, but transfection results shows no signals after various attempts. The...

31 December 2016 696 4 View

How to prepare gel filtration column manually?

Can anyone advise me regarding the manual packing/ preparation of gel filtration column using Dextran resin G-75. Details about the column, methods and materials?

05 June 2016 4,048 6 View

Why is His-tagged protein not visible in western blot when recombinant protein gets expressed in the same molecular weight every time?

My His-tagged recombinant protein (MW-50 kDa) is fully over-expressed in desired molecular weight on SDS-PAGE. Before purification i need to confirm it through western blot. After various attempt...

03 April 2015 9,480 26 View

Problem regarding pull down assay. Can anyone lend their expertise?

I am trying to identify interaction partners of my recombinant protein. I set up the pull down assay including my recombinant protein and negative control i.e. having same tag which is present in...

06 July 2014 305 3 View

Can anyone help with bioinformatics tools for protein-protein interaction?

Suggestion for the bioinformatics tools which we can use to study the protein-protein interaction, protein-protein complex interaction study and interaction of interacting partners and recombinant...

03 April 2014 1,427 10 View

Any advice on rice sample preparation for SDS PAGE analysis?

I want to run an SDS PAGE of rice crude proteins. I used a grinding method with liquid nitrogen, but was unable to make a good sample that I could use for SDS PAGE. Is there any other suggested...

10 November 2013 8,910 5 View

Can anyone share any protocol for the pull down assay for the recombinant protein?

I am trying to pull down the yeast proteins against my recombinant protein, but i am not sure about the protocol. Tried to search in internet but not found such protocol for the pull down.

09 October 2013 9,137 10 View

Multiple band in western blot?

I wanted to check for my target protein in crude extract of e coli cells. I cloned my protein with his tag. so i used anti his antibody following with secondary antibody. After developing I was...

08 September 2013 3,131 5 View

Geotechnical Engineering (Proceedings of the ICE) time review?

Hello everyone, I recently submitted an article to Geotechnical Engineering (Proceedings of the ICE), and the current status has been listed as "EiC Pre-assessment: Ready" for the past 20 days. I...

10 August 2024 6,493 1 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

Pls send me IEEE conference paper template if anyone have?

IEEE Conference template

02 August 2024 8,762 2 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

What should a Mechanical Engineering PhD scholar focus on during their PhD to enhance their chances of securing a postdoctoral position?

29 July 2024 7,714 4 View

May I know the exact Quartile of the journal- Advanced Engineering Materials (Wiley) for material science category?

In some data sources it has been grouped in Q1 and some shows it is Q2.

29 July 2024 4,227 2 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

I am working on my Master's thesis on the biogeography of the genus Ruagea and I would like to ask, could someone help me to check whether my result?

I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...

28 July 2024 2,979 1 View