No Fluorescence signals in GFP based imaging and expression?

We have prepared a construct using GFP sequences in middle. Although sequencing analysis confirmed the positive clone, but transfection results shows no signals after various attempts. The...

31 December 2016 568 4 View

How to prepare gel filtration column manually?

Can anyone advise me regarding the manual packing/ preparation of gel filtration column using Dextran resin G-75. Details about the column, methods and materials?

05 June 2016 3,902 6 View

Why is His-tagged protein not visible in western blot when recombinant protein gets expressed in the same molecular weight every time?

My His-tagged recombinant protein (MW-50 kDa) is fully over-expressed in desired molecular weight on SDS-PAGE. Before purification i need to confirm it through western blot. After various attempt...

03 April 2015 9,363 26 View

Problem regarding pull down assay. Can anyone lend their expertise?

I am trying to identify interaction partners of my recombinant protein. I set up the pull down assay including my recombinant protein and negative control i.e. having same tag which is present in...

06 July 2014 184 3 View

Can anyone help with bioinformatics tools for protein-protein interaction?

Suggestion for the bioinformatics tools which we can use to study the protein-protein interaction, protein-protein complex interaction study and interaction of interacting partners and recombinant...

03 April 2014 1,309 10 View

Any advice on rice sample preparation for SDS PAGE analysis?

I want to run an SDS PAGE of rice crude proteins. I used a grinding method with liquid nitrogen, but was unable to make a good sample that I could use for SDS PAGE. Is there any other suggested...

10 November 2013 8,783 5 View

Can anyone share any protocol for the pull down assay for the recombinant protein?

I am trying to pull down the yeast proteins against my recombinant protein, but i am not sure about the protocol. Tried to search in internet but not found such protocol for the pull down.

09 October 2013 9,033 10 View

Multiple band in western blot?

I wanted to check for my target protein in crude extract of e coli cells. I cloned my protein with his tag. so i used anti his antibody following with secondary antibody. After developing I was...

08 September 2013 2,982 5 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

No title

02 March 2021 324 2 View