Gaurav Chhetri

9 Questions 111 Answers 0 Followers

Questions related from Gaurav Chhetri

No Fluorescence signals in GFP based imaging and expression?

We have prepared a construct using GFP sequences in middle. Although sequencing analysis confirmed the positive clone, but transfection results shows no signals after various attempts. The...

01 January 2017 642 4 View

How to prepare gel filtration column manually?

Can anyone advise me regarding the manual packing/ preparation of gel filtration column using Dextran resin G-75. Details about the column, methods and materials?

06 June 2016 3,989 6 View

Why is His-tagged protein not visible in western blot when recombinant protein gets expressed in the same molecular weight every time?

My His-tagged recombinant protein (MW-50 kDa) is fully over-expressed in desired molecular weight on SDS-PAGE. Before purification i need to confirm it through western blot. After various attempt...

04 April 2015 9,440 26 View

Problem regarding pull down assay. Can anyone lend their expertise?

I am trying to identify interaction partners of my recombinant protein. I set up the pull down assay including my recombinant protein and negative control i.e. having same tag which is present in...

07 July 2014 264 3 View

Any good workshops or conferences on protein-protein interactions?

Any information regarding any upcoming good conferences or workshops in India or abroad?

05 May 2014 6,860 4 View

Can anyone help with bioinformatics tools for protein-protein interaction?

Suggestion for the bioinformatics tools which we can use to study the protein-protein interaction, protein-protein complex interaction study and interaction of interacting partners and recombinant...

04 April 2014 1,387 10 View

Any advice on rice sample preparation for SDS PAGE analysis?

I want to run an SDS PAGE of rice crude proteins. I used a grinding method with liquid nitrogen, but was unable to make a good sample that I could use for SDS PAGE. Is there any other suggested...

11 November 2013 8,857 5 View

Can anyone share any protocol for the pull down assay for the recombinant protein?

I am trying to pull down the yeast proteins against my recombinant protein, but i am not sure about the protocol. Tried to search in internet but not found such protocol for the pull down.

10 October 2013 9,104 10 View

Multiple band in western blot?

I wanted to check for my target protein in crude extract of e coli cells. I cloned my protein with his tag. so i used anti his antibody following with secondary antibody. After developing I was...

09 September 2013 3,084 5 View