Any extraction buffer for the soil RNA?

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Ian Hu

good old Trizol.

Lucy Kamau M.

You can treat the soil sample with mild NaCl/KCl say 0.7M/ 250mM before extracting with TRIzol as usual

Aqleem Abbas

how to prepare 0.7M/250mM, Need to sterilized it or not

Have you tried ???

Yes. Filter sterilize the buffers

Please write the whole protocol including epindorph tubes size and pellets plus chemicals used.

Mushtaq Ahmad

http://www.rombio.eu/vol19nr1/1%20lucr%202%20Canul-Chan%20rec08.09.2013ac%2008.10.pdf