Any experiences with synthetic spike-in controls for microRNA analysis using qPCR?

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Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

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Ulrike Jung

qPCR means stem-loop primer TaqMan RT-PCR? Because qPCR is a very general term and does not explain what you actually measure.

Besides that I can recommend the Applied Biosystems miRNA TaqMan assays, they have the synthesized mature miRNA available too (call them pre-miRNA for some odd reason though).

Johannes Denk

Yes, i'm using stem-loop primer TaqMan RT-PCR! It seems that applied biosystems is offering this kind of synthetic microNA as microRNA "mimics" as well! Thanks for this hint! But i couldn't find any manual which describes the quality control in detail - any further suggestions!?