Any different between method extraction DNA for fungi and bacteria
Losing lives for covid-19 is sorrowful. It could be avoided. People do participate and help spreading the virus whether intentionally or unintentionally by violating rules. I'm astonished to...
28 December 2020 2,168 66 View
28 December 2020 5,343 56 View
One of the goals of the scientific platforms, including Research Gate, is to help researchers to update their knowledge regarding the recent developments in research in their area of study. We...
08 October 2020 9,271 24 View
It is a problem when we make a search for something, theoretical or practical, related to translation "between two languages" and then we come out with the list of attractive titles and then we...
23 September 2020 1,365 8 View
In fact, this question is raised by a student. Our study materials for translation include both theoretical and practical considerations. The question is "do we need to...
21 September 2020 1,475 8 View
What are the most important pillars of negotiation between Saudi Arabia and Iran? What is the position of the international powers towards them? To what extent will these negotiations succeed in...
01 January 1970 1,948 4 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
Are there any statistical methods to justify your sampling technique using SPSS or AMOS?
05 August 2024 9,153 4 View
There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...
05 August 2024 4,945 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View