Hello all,
1. Is there anything database that helps in the identification of tumour types and relevant cell-lines where CCDN3 mutations are observed? I‘m interested in the CCND3 mutations that accrue around the phosphorylation motif at amino acid T283.
2. We have a drug that causes CCND3 degradation in cell lines. Is there any experiment to confirm our findings? Eg: looking further at expression level of genes that are activated by CCND3?
Thanks
Lax
In Cosmic you will find a comprehensive overview of somatic mutations that have been observed in CCND3:
http://cancer.sanger.ac.uk/cosmic/gene/analysis?ln=CCND3
You can then query each mutation and see in which "sample" it has been found. There does not seem to be any mutation reported in T283, but there are some in the neighborood. For example P284S:
http://cancer.sanger.ac.uk/cosmic/mutation/overview?id=144647
Here you can click on the sample links:
http://cancer.sanger.ac.uk/cosmic/mutation/overview?id=144647
In that example, you have 4 different samples with this mutation and you can see in which type of cancers they were observed. Only one sample is "cultured" (ie a cell line), it is NU-DHL-1:
http://cancer.sanger.ac.uk/cosmic/sample/overview?id=1629931
Here if you want more knowledge on what this cell line is you better go out of Cosmic and go to our Cellosaurus (there is no direct link in that direction), So just do a search of the sampleID or the name and you will find:
http://web.expasy.org/cellosaurus/CVCL_1876
You could automatize the whole process of retrieving all cell line samples with mutations in CCDN3 in Cosmic using their "Cosmic MART" application, but if you are only interested in mutations in the vicinity of T283, it will be much faster to go through the steps described above manually as there is only a limited number of mutations described in that gene.
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