Lax Lee

10 Questions 6 Answers 0 Followers

Questions related from Lax Lee

Any database showing cell lines and CCND3 specific mutation status?

Hello all, 1. Is there anything database that helps in the identification of tumour types and relevant cell-lines where CCDN3 mutations are observed? I‘m interested in the CCND3 mutations that...

10 October 2016 3,177 1 View

Human BCL2 protein with N-6x His tag purification- expression and purification help?

Hello, I have been trying to express and purify full length human BCL-2 protein in E coli. BCL-2 cDNA determined to be correct were introduced into Gtaeway pDEST 17 vector to allow for the...

07 July 2016 4,638 4 View

Is it possible to treat cell lysate with different drugs?

Hello all, i wanted to know whether its possible to culture a particular mutant cell line which over expresses a protein X (proved), lyse them and treat the lysate with varying concentrations of...

05 May 2016 4,794 6 View

Can we test Cam protein, protein X and protein Y binding???

I have CaM bound sepharose beads. I also have a Purified protein 'X' and one of my cell line over expresses protein 'Y'. I need help with the experiment planning to look for these protein...

05 May 2016 5,009 3 View

Cell seeding in 96 well and 6 well plate for cell line and protein assays?

I did my cell line assay in 96 well plate and wanted to do the corresponding protein assay in 6 well plate. For 96 well plate the seeding density i used was 40,000cells / well in 100ul final...

04 April 2016 8,524 1 View

How much concentration of calcium to add in cell line???

For one of my cell culture assays, i need to add additional calcium. The culture medium i use has 1.6mM calcium. Do I need to count this. I am interested i effective concentration suitable for...

04 April 2016 9,984 1 View

Cell line calculation??

I have used 40,000 cells/well for my cell line cytotoxicity assay in 96 well plates. the volume was 60ul. i have used 10ul of 100uM concentration of drug. Now I want to do immunoblotting of the...

03 March 2016 4,111 2 View

Need to conform the interaction of protein A and B?

Hypothetically i need to look at whether my protein say A in the presence of a drug destroys protein B in cell lines. I have in vitro cell lines assays to show this but I need to substantiate...

01 January 2016 5,864 2 View

Pull Down protein interpretation?

I am doing pull down using His tagged protein A (28kDa) and an untagged protein B (47kDa). I incubate the proteins in presence and absence of a drug. In presence of drug protein A should...

01 January 2016 252 5 View

Can you give some suggestions on Pull down experiment planning?

Hello, I am a intern, very new to pull down assays. I am planning a His- pull down experiment. I have His tagged Protein A and an untagged protein B. I want to know whether they interact each...

12 December 2015 7,372 6 View