Zahra Zangishei

5 Questions 10 Answers 0 Followers

Questions related from Zahra Zangishei

how to best store collected tissues and maintain reproducibility between laboratories?

I am going to store my plant tissues for further experiments on (mRNA/miRNA-seq AND qPCR) later in another laboratory. Due to transportation process of my samples, so I am worry about maintaining...

06 June 2019 5,436 4 View

Any comment on my RNA non-denaturing gel?

I checked the quality of total RNA of tomato tissues following Trizol extraction on non-denaturing gel. I am planning to submit my samples for small RNA-Sequencing. What caught my attention is...

11 November 2017 2,549 2 View

How about the performance of BGISEQ-500 vs Illumina HiSeq2500 sequencing platforms for small-RNAs sequencing?

Hi everybody. As I am at the critical step of my project, I wonder which sequencing platform may results in more quantified and valid small -RNA Seq data. Today, the Illumina series are the...

10 October 2017 7,988 4 View

Are rep.s needed for small-RNA/RNA seq libraries?

In more than several papers studing small-RNA/RNA seq I can see for each treatment just one library has been prepared that consists of e.g. 12 pooled biological RNA samples, and no replication...

10 October 2017 6,156 2 View

Is there any reliable computational miRNA binding site prediction programs?

I want to find probable miRNAs bind to and interact with my gene of interest. So, I think of predicting the putative binding sites of miRNAs on my target sequence. Is there any reliable...

01 January 2017 2,830 10 View