Any clue on bacterial necrosis/apoptosis speed?

More Chiththaka Chaturanga Bandara's questions See All

What are the alternative methods to measure the filler dispersion in a rubber composite other than dispergrader?

Hi everyone, I would like to seek some answers from you for my question above mentioned. If you know something let me know. Cheers

23 February 2024 2,397 2 View

I want to know how to prepare solution that use for autozero and base collection in the uv spectrophotometer?

I have dissolve my extracts using DMSO and I got absorbance that increase with the concentration. I have dough when I prepare measurements in the spectrophotometer I only use 2 sample of DMSO and...

12 February 2024 2,411 0 View

Create a Use Case Diagram that illustrates the use cases for the following Banking Application?

Customers are downloading and using the Banking App. The Bank is going to provide information that feeds into the Banking App, like transactions and account balances. The Banking App is allowing a...

26 April 2023 9,172 1 View

Which programs are to be used to register 2D image onto a 3D image data set?

I am trying to register a light micrograph onto a 3D CT data set, as I want to extract the corresponding image from the CT data set. Is there any software or program can do this accurately? I am...

21 February 2021 8,564 3 View

Are there any Statistical Quality Controlling researches based on the survey?

I want to know about uses of Statistical Quality Controlling techniques in the industries. And I hope to study this matters on survey with questionnaire.

26 December 2020 3,476 3 View

Looking for a Free, High Performance Computing Facility?

Dear All, I'm looking for freely available HPC facility to do a MD simulation for a short time period. Is there anyone, who can help by providing the access to the HPC to do the simulation.....

30 September 2019 7,566 3 View

Can you change the desorption temperature by changing the pressure?

When desorbing water from saturated silica gel, can you reduce the desorption temperature by decreasing the pressure of the gas around the adsorbent (silica gel) ?

30 May 2019 3,177 3 View

What is the best reliable primers set /protocol for SYBR Green I based quantitative detection of Dengue and nested PCR for sero-typing?

Presently I'm using Lanciotti et al., protocol for detection of Dengue using SYBR green1 PCR and I need to extend it for quantification of copy number / viral load in patient serum samples. I...

19 September 2018 8,872 1 View

ANN does not process information.It only represent information. ?

Are there any research improvements related to attention, emotions, motivation, pleasure, consciousness and personality developed using ANN.

21 August 2018 9,187 4 View

Possible Methods of evaluating Financial Reporting Quality ?

The study is to evaluate FRQ with the compliance level of Qualitative Characteristics (QC) . Using annual reports of listed entities,Beest, Braam, & Boelens, 2009 quality index is to used to...

22 May 2018 5,575 7 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View