Any alternative for cellosolve for mounting Culex genitalia?

17 May 2021 0 10K Report

I am preparing slides for the genitalia of male mosquitoes of the Culex pipiens complex. I read it is used cellosolve to dehydrate the genitalia. Any alternative to replace it?

Badges
Science topic

More Kevin Rucci's questions See All

How to identify wetland area in Landsat imagery?

The indexes NDWI and MNDWI are use to identify waterbody. Can I use these index to identify wetland also? Thank you for advance.

25 July 2024 6,242 2 View

Im trying iodination of an arene, but only getting black precipitates, is there anyone with iodination experience who can advise?

Organic Synthesis. My student has been trying iodination of arenes (our goal is to iodinate p-phenylenediamine) following these literature procedures: 1. Synthesis 2004, No. 11, 1869-1873 using...

16 July 2024 6,630 1 View

Is there a way to calculate cell density and thus cell count from confluency for RPE-1 cells?

I am growing cells in a 24 well plate. I do not have access to a microscope with a camara, so I have to calculate the number of cells in the wells manually. I can get the confluency pretty easily,...

04 July 2024 9,622 0 View

Why am I seeing no color change of the positive control cell death assays?

I am currently running Cell Death ELISAs, using the Roche Cell Death Detection ELISA kit. My samples are from the supernatant of bacterially infected primary canine cells. I am seeing no color...

02 June 2024 8,682 1 View

Latest Advancements in Minimally Invasive Heart Surgery ?

What are the latest advancements in minimally invasive heart surgery techniques, and how do they compare in terms of patient outcomes and recovery times to traditional open-heart surgery?

16 May 2024 3,336 2 View

What would be some simple definitions to give to coaches and athletes on force platform metrics?

Jump Momentum Force at min Displacement Avg Propulsive Force Braking RFD Avg Braking Force

12 May 2024 768 0 View

¿Como puedo transformar una variable donde predominan los ceros y también hay valores altos dispersos?

Tengo una columna de la variable de "precipitación" donde predominan los ceros y hay valores altos. No he podido transformarla para obtener una distribución normal, ya he usado métodos de log,...

05 April 2024 8,427 1 View

Is it necessary to include an FMO for 7-AAD dye in your Flow Cytometry experiment? If not, why?

I'm optimizing a flow cytometer experiment and one of my three stains is a 7-AAD Live/Dead stain. The other stains in my experiment are staining for CD34+ and CD45+ cells. Is it necessary to...

01 April 2024 3,060 0 View

Would you have a simple Protocol for Flow cytometry cross match test (in flow tubes)?

The cross-match test is an in vitro test to determine the presence of anti-lymphocyte antibody to donor cell antigens (lymphocytotoxic antibody) in serum of an individual with preformed...

01 April 2024 3,203 1 View

Has anyone observed an injury caused by a spiny dogfish sting resulting in isolated muscle atrophy in human?

Fisherman stung while extracting hook from fish.

19 March 2024 535 0 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

Is there any psychological intervention or Therapeutic protocol for "PGAD" ?

"persistant genital arosal disorder"

24 July 2024 4,303 0 View

Is there any psychological intervention or Therapeutic protocol for "PGAD" ?

"persistant genital Arosal disorder"

22 July 2024 2,930 2 View

Why should women want to look at their unaroused genitals?

Unlike a boy’s very obvious penis, which he knows is pleasurable to touch and fondle, a girl’s genitals are hidden, mysterious, remote. I find it remarkable (and disturbing) that many women have...

17 July 2024 3,874 0 View

Is there any possibility to remove pesticides from fruits & vegetables,other than vinegar &which fruits &vegetables do not dehydrate well for storage?

Is there any possibility to remove pesticides from fruits and vegetables, other than vinegar and which fruits and vegetables do not dehydrate well for storage?

02 July 2024 396 1 View

What can cause that mosquitoes from Rockefeller reference strain (Aedes aegypti) spend too much time in larval stages?

We have been rearing Rockefeller mosquitoes for many years in our laboratory without any problems, but lately, in spite of good viability of eggs and unchanged environmental conditions (27°C,...

20 June 2024 3,515 2 View

In the case of Collagen Cell Carrier® membrane 34 mm for 6 well cell culture plate (Viscofan), can you suggest a paraffin embedding protocol?

I am intrested in the preparation of collagen carriers, dehydration, paraffin infiltration, and paraffin embedding. Thanks in advance.

05 June 2024 3,831 0 View

Why do many women talk of lovemaking to rather than sex?

It is difficult for most males to comprehend that females are not aroused by seeing male genitalia. Some males never come to comprehend this. (Alfred Kinsey)

11 May 2024 5,981 0 View

Air bubbles forming underneath tissue during dehydration step of IHC?

Hi all, I've processed my own IHC for many years and never run into this problem before, but the last few times I've tried staining, I've had the same issue. I'm wondering if it's a reagent...

08 May 2024 3,979 1 View

Can 100% alcohol (95% EtOH, 5% methanol/isopropyl alcohol) be used for bacterial SEM dehydration?

I am preparing a protocol for SEM of a biofilm, but do not have easy access to 100% ethanol for the dehydration steps. Can 100% ethanol be substituted for 100% alcohol (95% EtOH, 5%...

07 May 2024 1,515 3 View