How to calculate theta, theta prime, coalescent depth and minimum interspecific distance?

I am working on DNA barcoding of two closely related plant species. I have used 5 marker regions for my analysis. How to calculate theta, theta prime, coalescent depth and minimum interspecific...

10 November 2016 9,669 3 View

Input matrix for Splitstree for Dominant markers using binary data ?

I am working on conservation genetics of some alpine medicinal plants. I have used some dominant markers and co-dominant markers. I am unable to construct network tree by using Splitstree. Can...

04 May 2016 928 4 View

What are "Unverified" sequence in Genebank? Can I take those unverified sequences from Genebank for my phylogenetic analysis?

I am working on molecular phylogeny in the order level. When I am extracting the sequences from NCBI Genebank, I found there are lot sequences marked as unverified. For concratenation of multiple...

04 May 2016 6,268 7 View

How much impact factor do we need to get a PDF in plant science in European country or in USA??

Impact factors are playing a pivotal role now a days in the age of competition. Good work or high impacts....??? How many modern techniques or simple yet novel breakthrough ....???? New idea or...

01 February 2015 8,537 2 View

How to deal with the problem in Internal transcribed spacer 2 amplification?

I am working on some plants from Valerianaceae family(angiosperms). I have already sequenced rbcl, matK and two inter-genic spacer regions. I want to amplify ITS1 and ITS 2 regions. Here,...

08 September 2014 3,814 8 View

Can you help me with the operation of Mr Bayes software and the procedure for Nexus file conversion?

06 July 2014 9,232 4 View

What are the steps before sequencing of a PCR purified product for rbcL, matK, psbA-trnH, trnL-F?

I have already amplified rbcl, matk, trnl-f, psba-trnh and ITS regions of my study plants. Now I will be doing sequencing of those regions. I have purified those PCR products by Quaquick gel...

06 July 2014 8,826 5 View

Can anybody help me with ITS 2 and ITS 1 amplification?

I got very nice bands with ITS 2 and ITS 1 in my study plant samples. I collected them from remote areas of India. Recently, in both ITS markers, I am not getting the bands. I have checked my...

05 June 2014 3,943 13 View

How can I generate a contig from raw sequence data?

If one gene is over 700 bp long then how to get the consensus sequence data from the raw sequence data? I have done the sequencing using both forward and reverse primers. Also, I want to know the...

05 June 2014 9,342 6 View

I got a peak at 422/423 nm in UV spectra. What is the size of my Ag nanoparticle?

Does colour and size of silver nanoparticles depend on each other? I used to get a peak at 430-450nm in UV spectra, but now I am getting a peak at 423nm. Sometimes I get double peaks (one small @...

05 June 2014 8,892 3 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

The Shannon's index is inversely proportional to Simpson's index (1-D)?

I'm having a problem with my bloxplot with Shannon's and Simpson's indexs. The value of Shannon for my Community 1 indicating higher diversity (in relation of the Community 2), but have lower...

26 February 2021 3,033 9 View