Emma Aneheim

3 Questions 4 Answers 0 Followers

Questions related from Emma Aneheim

Does anyone have Cyclone Plus user instructions?

We have an old Cyclone Plus standing i our lab that never has been taken in use and hence, noone knows what to do with it. I would really like to get it up and running but I don't know where to...

10 January 2020 2,913 0 View

Why won't my HiTrap desalting column perform as well as its NAP-5/10 counterparts in purifying radiolabeled antibodies?

Same media in both columns (GE Sephadex 25). Pre-treated with BSA in the same way. Flowrates within limits stated for he HiTrap. Still bad perfomance. The outcome is a pure product but a lot of...

28 January 2015 5,480 9 View

Any advice on contamination during gel filtration of Herceptin?

I'm using commercial Herceptin for some experiments and when running FPLC (column superdex 200, PBS mobile phase, eluting 0.5ml/min) on my samples I find, besides the antibody peak (UV 280nm) at...

29 November 2013 1,694 3 View