Did anybody worked with EphA family protein expression? MW 130kDa . I am not getting any expression. Do you use different lysis buffer or RIPA?

I am working on EphA2, pEpA3 expression with different cell types (MD-MBA231, MOLT4) for the expression of EphA family proteins, They are of high molecular weight 130 kDa. I use RIPA buffer and...

01 December 2020 8,610 1 View

What statistical test should I use if I want to compare values of temperature taken at aural, forehead and inner wrist ?

I basically want to see if there is a difference between these sites. Especially between the forehead and inner wrist as many patients are not very happy with temperature reading taken at the...

05 October 2020 3,151 12 View

Need weather and metrological data for forecasting or predicting the agriculture in Saudi Arabia?

Need weather and metrological data for forecasting or predicting agriculture in Saudi Arabia? This is for the purpose of one of the project by my fellow researchers. Kindly share the same.

11 August 2020 8,163 4 View

What are the high impact journals available in sustainability and sustainable development domain?

What are the high impact journals available in sustainability and sustainable development domain?

24 June 2020 3,979 11 View

What are the COVID-19 papers retracted from journals?is it clinical, treatment, diagnosis or AI based prediction? Why?

What are the COVID-19 papers retracted from journals? is it related to clinical, treatment, diagnosis or AI-based prediction? Why

07 June 2020 7,301 3 View

Any suggestions for SCOPUS Indexed journal for social science field, specifically for education?

I'm looking for any SCOPUS indexed journal to send my article. Social science specifically in education field with minimum waiting period for the acceptance. I did contacted some journal editors...

20 April 2020 8,242 1 View

Hydroxychlorine was proposed as drug for corona viral disease (COVID-19). Is it good for treatment?

It might induce immunosuppression.

03 April 2020 3,740 4 View

Quantitative & Qualitative perceptive. (mix mode)?

Dear All, My research topic is socio-economic impact of employment of Filipinos Maids in Singapore. My research will be both Quantitative & Qualitative perceptive. (mix mode) My question:...

19 October 2019 5,959 8 View

How can we determine the sample size from an unknown population?

Please provide example too, im new ...

09 April 2019 5,724 4 View

Transformed colonies with GFP showed Faded Fluorescence ?

Transformed Yeast colonies with a Plasmid having GFP, showed faded Fluorescence. It was light yellowish in colour rather than the usual Green Colour . What could be the reason ? The Microscope was...

28 December 2018 7,516 4 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

Which SAR dataset to use for generating coherency matrix and performing target decomposition (Description) ?

Working on chandrayaan-2 DFSAR data, there are three datasets available: 1) Slant range image data product: The slant range complex image file. Each pixel is represented by two 4-byte floating...

02 March 2021 8,481 3 View

Where can I find atmospheric measurement profiles (temperature, density, etc.) between 300-800 km altitude?

I am investigating thermospheric mass density profiles between 300-800 km altitude. I would like to compare the variability with other parameters, including temperature, electron content,...

01 March 2021 5,902 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

How can we limit artifacts caused by rotation in fiber photometry?

We are trying to run fiber photometry experiments for long time scales, but we get these sinusoidal artifacts that come up. We think they may be caused by twisting of the fiber optic subject...

25 February 2021 568 1 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

How to clean up TEM grid with plasma cleaner?

I need to clean the hydrocarbon contamination on the carbon substrate of TEM grid. However, I heard that substrate could be broken by the powerful plasma. My substrate is pure carbon, with ~3nm,...

23 February 2021 3,262 5 View

Are there methods for automatically mapping floodplains, fans and terraces?

I want to map these river geoforms with as little subjectivity as possible. I have a high resolution DEM generated by drone data.

22 February 2021 7,800 3 View

Covid 19 Pandemic ' impact on digital transformation?

Covid 19 Pandemic and digital transformation

22 February 2021 3,351 5 View