Any advice/information/recommended literature on the ELISA Technique?

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Jamil Mourad

Mirza kindly find attached this reference

M. Mirza Nuryady

wow thankyou so much Wathsala Wijayalath and Jamil Mourad you all so kind hearted. Thankyou so much it's really helpfull

José Roberto Mineo

Mirza, I agree with Jamil, the ELISA Guidebook 2nd ed.pdf [John R. Crowther, Springer Protocols - Methods in Molecular Biology 516] will give you all necessary information to validate your immunoassay. Good luck.

Subhajit Dasgupta

Best thing is to follow any company literature; so you will get the working protocol. Check with E bioscience or BD Bioscience ELISA kit ; you will get ELISA protocol to work with. They will provide with sandwich ELISA protocol which you can set with your antibodies and antigen.

your welcome Mirza