Hello Ramirez

It is not a new subject. There are a lot of known plants which have antimicrobial effects.

I suggest another subject for discussion:

New and different methods for increasing antimicrobial effect (Nano- thech; Extraction methods, ...)

Regards

Dehghan

If there are many plants with antimicrobial effect, why there aren't many antibiotics derivate to that?

Hi Ramirez

I don't have good information about European or American countries, but in Asian countries TRADITIONAL AND HERBAL MEDICINE are custom methods for treatment.

Some of good herbs with antimicrobial effect are Eucalyptus, Garlic, Thyme. You need to search them in internet and find a lot of commercial products.

Good Luck

Dehghan

Roman - good question as to why there are not more natural antibiotics on the market. In Europe, chamomile is used as topical antiseptic, and thyme is used in a number of products. But developing a new drug from an isolate is very costly, and it may be more challenging to get a patent on it (thus, less enticing for a company to work on it). If you look at plant-derived antimicrobials, they often have a fairly good efficacy against gram+ bacteria, but may lack activity against some of the more challenging gram- strains. So there are also advantages in terms of broader spectrum and better efficacy/bioavailability for the "regular" antibiotics on the market, most of which come from natural sources (yeasts, fungi, microbes) as well.

I am very interested in studying the antibacterial activity of essential oils of forestery species in our laboratory and we were able to extract more than 32 species and I am currently asking for a collaboration with a laboratory specializing in the field of study antibacterial activities of essential oils

Collegues we must work together against bacterial resistence. I propose a more open communication to assess the procedures and techniques that we used in our experiments.

I like this discusses and also the medicinal and aromatic plants

It’s not a new issue but so far only a few percent of plant species were screened against bacteria and fungi. Bioactive compounds are still the major source to finding the new active compound against these organisms. If you are going to discuss deeper you can talk about chemical structures and mode of action of these compounds.

Stefan is correct regarding the 'patent' as being the prime motivator for any 'pharma'-type company to invest in the research of antimicrobial activity of plant extracts. At my university, we follow the general pattern established by Eloff and the Phytomedicine Programme at the University of Pretoria in South Africa. Using the leaves of trees is a sustainable way of capitalising on the discovery of natural products with any biological activity ... not just antimicrobial! Some Australian plants exhibit a range of previously undescribed (especially terpenoid) metabolites that show promising activities.

Thanks by your comments!! Please comment our experiences in order to standarize evaluation techniques of plant extracts and essencial olis. I´m from Colombia and I see that this discussion is important for collegues around the world.

collegues, due this time i'm doing the research about antibacterial activity from plant, however, just like Mr. Stefan gafner said, this essential oil mostly effective only against gram + bacteria.

can you suggest me how to increase the effectivity of the essential oil to inhibit the gram - bacteria?

Pramono, antimicrobial effect in many proportion is due to the extraction technique (solvents, times, etc) Is difficult increse the effectivity of the especific essential oil because in such oil there is a determinate number o class of mollecules and nothing more. Maybe if you mix different oils could to result in a additive effect but too in an antagonistic effect. I recommend try with another kind of plants making screenings.

Roman, thank you for the response, just like you your suggestion my current project are about evaluation of chemical composition and biological properties from several kind of traditional Thai herbal plant, i wish i could get a good result about it

Pramono, I think if we focus on how the herbal plants are combined during use and try to isolate the active principle formed by the combination of different plants (rather than isolatings seemingly active principles from the different plants) we may be on the right track towards getting active principle that would be active against resistant bacterial strains. i believe combination of the different plants would result in chemical reactions leading to the formation of a different active principle, which accounts for the biological activites of some of this herbal products.

I am in support of you current research project. Keep it up.

An antimicrobial is a substance that kills or inhibits the growth of microbes such as bacteria, fungi or viruses.

Some plants have been investigated scientifically for antimicrobial activity and a large numbers of plant products have been shown to inhibit the growth of pathogenic microorganisms.

In recent years, workers in the medicinal field returned back to nature particularly in the use of medicinal plants to treat human ailments. This new trend was supported by: The recent WHO orientation strategy that embarked on examination of the historical position of traditional medicine at their intersections with the development and modernization of a biomedically-based health care system (WHO, 2002; Dole, 2004).

hi i am very intrest to join this group

You are welcome !!!

is there any suggestion for doing MIC value testing if our sample is limited, because if by agar dilution method, i try to calculated before i do the test, i'll need a lot of sample, can somebody help me?

thank you for your suggestion Kateryna Kon, i'll try it.

antibiotic powder, I use it.

It depends of bacteria if it is grampositive or gramnegative.

Hi Kateryna kon, bacteria like leptospira grow only in broth. So assays will be done in broth dilution method only. We use antibiotics in powder form to study. For other bacteria it is easy to study the readings but in leptospira like organisms it is some what difficult. But go through the paper which is mentioned down which may be helpful to you. If you feel this is complicated then I can try to send you some more papers if you mention specific organism which you are interested to study

Broth Microdilution Susceptibility Testing for Leptospira spp.

Clinton K. Murray and Duane R. Hospenthal*

Infectious Disease Service, Department of Medicine, Brooke Army Medical Center, Fort Sam Houston, Texas

Received 29 July 2003/Returned for modification 13 November 2003/Accepted 20 January 2004

hi madam kateryan kon, you disscused regarding broth dilution method with small amount of active compounds. Can you know any published paper regarding this so that I can try for it.

hello!! I'm really interested in doing researches specially experimental research. I am a graduate of Bachelor of Science in Nursing here in the Philippines and I know that I could excel more in the field of research and discovery. I would like to ask if anybody out there, great scholars and researchers who needs a research assistant I am enthusiastically would really want to work for you. Here is my e-add: [email protected]. Thank you very much!

hi kateryna kon, I able to download the article but I am not able to download that book. I request you to send a copy to my mail ID which can be helpful for my study.

one more thing I want to know that I got 4 medicinal plant crude extracts which are active against 7 human pathogens but now I have very less amount of extract. What experiments can able to do now any suggestions

Hi kateryna kon this is my email ID

[email protected] , [email protected]

send the book to any one of this IDs

does anyone ever download this paper?

Tzortzakis NG, 2009. Essential oil: Innovative tool to improve the preservation of fresh produce—a review. Fresh Produce (Special Issue), 3: 87-97

if you have this paper, would you please share it with me...thank you

Thank you for the information Kateryna.