Antimicrobial activity , how do we perform antibacterial activity

More Wahiba Rached's questions See All

How to calculate electron and hole mobility for 2D materials (such as MX phases) using BoltzTraP code?

Hello all, actually I am looking to calculate the mobility for 2D materials using BoltzTraP code. If anyone know about it, I will be highly thankful.

21 January 2022 9,503 3 View

How to calculate the electron relaxation time at different temperature from BoltzTraP code ?

Actually, I try to investigate the thermoelectric effect on some highly spinpolarized compounds by means of Boltzmann theory within the relaxation time approach. First I have calculated the...

08 January 2021 8,106 3 View

What is the minimal thickness of thin film cristalline silicon?

Hi, I wanted to know the minimum thickness that can be obtained when depositing crystalline silicon (with a gap of 1.12 eV) and is it possible to obtain it using the PECVD technique (Plasma...

26 November 2020 9,237 20 View

What is the best statistical analysis I could use to assess intersectionality of multiple marginalized identity?

I am conducting a study on multiple marginalized identities and mental health outcomes. What would be the best data analysis I should use to assess the interaction between different variables...

08 July 2020 7,904 1 View

Are there any Arabic version scales on Barriers to mental Health services?

Dear Colleagues, I am looking for Scales in Arabic measuring barriers to mental health services.; Stigma towards mental illness, Coping with mental illness, and attitudes toward mental illness.

01 May 2020 3,054 1 View

Is there is a free program computing thermal property?

Actually, I need free software for calculating thermal properties of crystalline solids namely, lattice thermal conductivity, and minimum thermal conductivity.

29 April 2020 3,083 9 View

How can I conduct a campaign about fighting Islamophobia using the Social Media ?

Hi, I am a Social Work professor and I serve on the social action day event. I would like to engage my students in a campaign against Islamophobia, as a social justice issue. I need some tips on...

21 February 2020 9,691 5 View

Is there any good scale in Arabic/English about Violence against refugees?

I am conducting a new study about Syrian Refugee women and would like to measure the level and type of abuse/violence they experience in a host country. Please let me know if you know of any scale...

04 January 2020 1,060 0 View

Activated carbon

Can someone suggest a way to functionalize S- groups on activated carbon surface with strong bond give reference?

16 March 2017 8,879 2 View

What is the best efficient way to run IEF electrophoresis?

Hey I am planning to run a 1D electrophoresis, to separate proteins according to their IEF. I have the Novex invitrogen ph3-7 reagents here. What is the amount of protein I need to load into the...

15 November 2016 9,526 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

For systematic review data extraction, should I use ITT or PP analysis, and count all randomized participants or only those who completed the study?

I am conducting a systematic review and meta analysis; as I am extracting data I realized there is a consort diagram that shows the number of patients from randomization till end of the study. So...

01 August 2024 9,993 3 View

For the moringa oleifera extract using ethanol as the solvent,what is the alternative method to concentrate the extract other than rotary evaporation?

99% pure ethanol was used for maceration also alternate methods along with the temperature and time to concentrate the extract can be specified

31 July 2024 5,113 4 View

How do soil microbes affect plant health and productivity and how do plants interact with soil microorganisms and contribute to soil fertility?

31 July 2024 5,087 4 View

What is the role of decomposers in the cycling of matter in the biosphere and role do soil microbes play in plant nutrition availability and uptake?

31 July 2024 7,921 5 View

What are the factors that causes the sample to have higher efficacy at lower concentration?

We conducted an antibacterial study of a plant extract. Varying concentrations of crude extract were subjected to microbroth dilution assay. The result showed that only the lowest concentration of...

31 July 2024 8,666 3 View

Tole S.B.

Dear Wahiba,

Regarding the antimicrobial activity of plant extract which contains phenolic extract, firstly you do...

Disk diffusion method (Agar Cup - plate method ) which giving the antimicrobial potential of your phenolic extract by observing the zone of inhibition (potential of your drug to fight microbial organism).

For carrying this kind of activity you select any two gram positive, two gram negative and one fungal organism.

After observing the zone of inhibition, you carry the MIC (minimum inhibitory concentration) value of your phenolic extract for confirming the antimicrobial potential of your isolated phenolic extract from plant

Phil Geis

What do you want to accomplish? Just to show the presence of activity? For that, zone of diffusion should be enough.

Wahiba Rached

Thank you Dr. Phil

Thank you Dr. Tole