Monitoring degradation by SEC and SDS-PAGE is a good idea. I hope this helps you :

A study published in BioPharm International examined the impact of freeze-thaw cycles on monoclonal antibody (mAb) solutions. The researchers observed that multiple freeze-thaw cycles led to increased aggregation, detectable by SEC and particulate analysis. They also noted that smaller container volumes and slower thawing rates contributed to higher aggregation levels.

Impact of mhttps://www.biopharminternational.com/view/impact-manufacturing-scale-freeze-thaw-conditions-mab-solutionanufacturing-scale freeze-thaw conditions on a mAb solution

Incorporating heating into the protocol can further simulate stress conditions, offering a comprehensive assessment of antibody stability.

https://www.malvernpanalytical.com/en/learn/knowledge-center/insights/impact-of-freeze-thaw-on-monoclonal-antibody-solutions

https://www.bioprocessintl.com/fill-finish/evaluating-freeze-thaw-processes-in-biopharmaceutical-development-small-scale-study-designs

Nicolas Poirier , thanks for your quick response and great resources.

DEar Amy E Griffiths

give a look to this review

https://pmc.ncbi.nlm.nih.gov/articles/PMC7153844/

best

Manuele