We are looking to obtain monoclonal antibodies to the B16 melanoma cell line. Can anybody assist?
Dear Professor.
I suggest you to see this paper. It may help you;
http://www.ncbi.nlm.nih.gov/pubmed/6206164
Can anyone direct me to a database of mouse TCR V beta families?
03 April 2019 5,351 1 View
I have a series of peptide sequences and I'm looking to see if there is a consesnsus sequence or motif. What tools do you recommend?
06 July 2016 3,394 9 View
We are starting a project on peanut allergy. I'm looking for an appropriate commercial source of the peanut allergen extract as well as purified Ara h proteins, especially h1 and h2.
11 December 2013 10,133 7 View
An interesting result to share. In one of our projects, cancer cells exposed to cell targeting phage show quite elevated alamar blue activity without any change in cell proliferation after 24 and...
07 August 2013 6,045 2 View
We are looking to isolate mRNAs of proteins destined for the plasma membrane. Can anyone direct me to literature discussing any defining differences between mRNAs of these proteins as opposed to...
01 February 2013 5,565 0 View
Looking for a successful protocol to maintain confluent primary cell cultures at basal, non-dividing conditions for some time to allow testing the effects of cytotoxic drugs. I've found several...
11 December 2012 7,004 13 View
We are developing an ELISPOT to measure antigen specific mouse B cells. We are using a standard protocol in which PVDF microplates are activated with 70ul ethanol then washed. Antigen solution is...
09 October 2012 5,546 11 View
We are evaluating a new anti-cancer drug that, among other things, blocks cells in G2. We need to test its non specific effects on normal cells but in regular cultures these are also replicating...
09 October 2012 6,076 9 View
We have calibrated the cell inoculation number (in matrigel) and growth kinetics of the above tumors but the results are inconsistent. Indeed sometimes the "growth" is actually an...
08 September 2012 9,266 10 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,664 6 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...
21 July 2024 1,044 1 View
It was assumed that 4E8 T cells should be isolated and activated using plate-bound anti-human CD3, after which would be used for a genome-wide CRISPR screen. (Ref: Genome-wide CRISPR Screens in...
17 July 2024 3,371 0 View
Hi there, I'd like to use immunocytochemistry to determine a surface protein expression on mouse cell. The primary antibody I have is mouse anti mouse, and the secondary antibody I have is goat...
15 July 2024 6,008 5 View
I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...
15 July 2024 6,668 0 View
Dear all, I'm going to radiolabel antibodies. So first of all, I'll remove excipients using the centrifugation method with amicon. Once I'm done, how do I calculate/measure the concentration of...
14 July 2024 3,106 3 View