Can anyone direct me to a database of mouse TCR V beta families?
https://www.jci.org/articles/view/44752
I have a series of peptide sequences and I'm looking to see if there is a consesnsus sequence or motif. What tools do you recommend?
06 July 2016 3,294 9 View
We are starting a project on peanut allergy. I'm looking for an appropriate commercial source of the peanut allergen extract as well as purified Ara h proteins, especially h1 and h2.
11 December 2013 10,024 7 View
An interesting result to share. In one of our projects, cancer cells exposed to cell targeting phage show quite elevated alamar blue activity without any change in cell proliferation after 24 and...
07 August 2013 5,934 2 View
We are looking to obtain monoclonal antibodies to the B16 melanoma cell line. Can anybody assist?
04 May 2013 1,147 1 View
We are looking to isolate mRNAs of proteins destined for the plasma membrane. Can anyone direct me to literature discussing any defining differences between mRNAs of these proteins as opposed to...
01 February 2013 5,468 0 View
Looking for a successful protocol to maintain confluent primary cell cultures at basal, non-dividing conditions for some time to allow testing the effects of cytotoxic drugs. I've found several...
11 December 2012 6,895 13 View
We are developing an ELISPOT to measure antigen specific mouse B cells. We are using a standard protocol in which PVDF microplates are activated with 70ul ethanol then washed. Antigen solution is...
09 October 2012 5,450 11 View
We are evaluating a new anti-cancer drug that, among other things, blocks cells in G2. We need to test its non specific effects on normal cells but in regular cultures these are also replicating...
09 October 2012 5,990 9 View
We have calibrated the cell inoculation number (in matrigel) and growth kinetics of the above tumors but the results are inconsistent. Indeed sometimes the "growth" is actually an...
08 September 2012 8,936 10 View
Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?
03 March 2021 1,763 2 View
Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...
01 March 2021 6,953 1 View
Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...
01 March 2021 9,938 3 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View
Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...
25 February 2021 155 2 View
Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...
24 February 2021 1,825 3 View
Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...
24 February 2021 5,759 3 View
Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...
23 February 2021 7,701 2 View
Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...
23 February 2021 5,476 4 View
Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha
22 February 2021 3,891 2 View
