Can anyone direct me to a database of mouse TCR V beta families?
https://www.jci.org/articles/view/44752
I have a series of peptide sequences and I'm looking to see if there is a consesnsus sequence or motif. What tools do you recommend?
06 July 2016 3,394 9 View
We are starting a project on peanut allergy. I'm looking for an appropriate commercial source of the peanut allergen extract as well as purified Ara h proteins, especially h1 and h2.
11 December 2013 10,133 7 View
An interesting result to share. In one of our projects, cancer cells exposed to cell targeting phage show quite elevated alamar blue activity without any change in cell proliferation after 24 and...
07 August 2013 6,045 2 View
We are looking to obtain monoclonal antibodies to the B16 melanoma cell line. Can anybody assist?
04 May 2013 1,246 1 View
We are looking to isolate mRNAs of proteins destined for the plasma membrane. Can anyone direct me to literature discussing any defining differences between mRNAs of these proteins as opposed to...
01 February 2013 5,565 0 View
Looking for a successful protocol to maintain confluent primary cell cultures at basal, non-dividing conditions for some time to allow testing the effects of cytotoxic drugs. I've found several...
11 December 2012 7,004 13 View
We are developing an ELISPOT to measure antigen specific mouse B cells. We are using a standard protocol in which PVDF microplates are activated with 70ul ethanol then washed. Antigen solution is...
09 October 2012 5,546 11 View
We are evaluating a new anti-cancer drug that, among other things, blocks cells in G2. We need to test its non specific effects on normal cells but in regular cultures these are also replicating...
09 October 2012 6,076 9 View
We have calibrated the cell inoculation number (in matrigel) and growth kinetics of the above tumors but the results are inconsistent. Indeed sometimes the "growth" is actually an...
08 September 2012 9,266 10 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View
