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Questions related from Michael Firer
Can anyone direct me to a database of mouse TCR V beta families?
04 April 2019 5,312 1 View
I have a series of peptide sequences and I'm looking to see if there is a consesnsus sequence or motif. What tools do you recommend?
07 July 2016 3,341 9 View
We are starting a project on peanut allergy. I'm looking for an appropriate commercial source of the peanut allergen extract as well as purified Ara h proteins, especially h1 and h2.
12 December 2013 10,089 7 View
An interesting result to share. In one of our projects, cancer cells exposed to cell targeting phage show quite elevated alamar blue activity without any change in cell proliferation after 24 and...
08 August 2013 6,004 2 View
We are looking to obtain monoclonal antibodies to the B16 melanoma cell line. Can anybody assist?
05 May 2013 1,203 1 View
We are looking to isolate mRNAs of proteins destined for the plasma membrane. Can anyone direct me to literature discussing any defining differences between mRNAs of these proteins as opposed to...
02 February 2013 5,522 0 View
Looking for a successful protocol to maintain confluent primary cell cultures at basal, non-dividing conditions for some time to allow testing the effects of cytotoxic drugs. I've found several...
12 December 2012 6,954 13 View
We are developing an ELISPOT to measure antigen specific mouse B cells. We are using a standard protocol in which PVDF microplates are activated with 70ul ethanol then washed. Antigen solution is...
10 October 2012 5,507 11 View
We are evaluating a new anti-cancer drug that, among other things, blocks cells in G2. We need to test its non specific effects on normal cells but in regular cultures these are also replicating...
10 October 2012 6,039 9 View
We have calibrated the cell inoculation number (in matrigel) and growth kinetics of the above tumors but the results are inconsistent. Indeed sometimes the "growth" is actually an...
09 September 2012 9,098 10 View
