Antibodies for Cerulean/CFP that do not cross-react with GFP?

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Good proliferation markers for zebrafish?

Hi all, suggestion for antibodies labelling proliferation that work well on zebrafish tissue? I ve used pH3 but I want something labelling more than just one phase (or labelling longer phases such...

25 April 2024 7,182 3 View

Can I compute the average or difference between different ERP components from different datastest in eeglab?

EEGLAB has the option to SUM/Compare ERP components or data channels for different datasets. In the case of components, I would like to compute the average of different components from different...

17 June 2022 5,990 2 View

How can I avoid having sign issues when using sum/compare ERPs in EEGLAB?

I want to compute the average and then difference of the ERP components using the pop_comperp() function in EEGLAB but I realized that I have some issues with the signs. The ERP data for some...

16 June 2022 9,331 3 View

Loading of the same factor in more than one components, should i delete them?

I'm using the big five personality inventory in my questionnaire. In the factor analysis items of the Big Five appear to more than one component. I know that it is better to get rid of the items...

14 April 2020 9,918 3 View

BrdU staining without HCl?

Hello, I am working on the enteric nervous system of adult zebrafish and I am trying to do an EdU-BrdU chase on a GFP transgenic line and the HCl treatment kind of destroys the tissue...

29 January 2020 8,298 7 View

Does anyone have the 1970 Muir paper on SEM EDS on fossil wood?

Dear all, I am looking for an old paper and I would be grateful if someone has it and is kind enough to send it to me. Muir, M. D. (1970)"A new approach to the study of fossil wood", Proceedings...

15 September 2019 2,734 9 View

Do you know papers at Internal Audit Effectiveness in Public Sector?

Please share papers about internal audit effectiveness at public sector published the last five years.Thank you.

23 September 2018 1,087 4 View

Why did my mice die after stereotaxic surgery?

I performed yesterday 6 subretinal injections using Isofurane as an anesthetic. The surgery lasted something less than an hour, isoflurane was set to 2% and mice were treated with Metamizol and...

06 April 2018 393 3 View

Automated earth weather stations (AWS) for the recording of environmental parameters of cultural heritage sites

We are looking for papers that deal with the use of automated earth weather stations (AWS) for the recording of environmental parameters of cultural heritage sites or palaentological sites.

22 May 2017 2,841 4 View

I am interested in research on requirements for dementia friendly hotel or dementia friendly tourism. Any suggestions?

I am working on a project and I am looking for research/ projects / reference or papers on hospitality for dementia or dementia friendly tourism.

01 November 2016 7,553 4 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Could dyes amplify the spectrum of light to a specific wavelength?

I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...

05 August 2024 3,290 1 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

Is it necessary to attach fluorophore at 3' end in MIC RT PCR machine for Dual hybridization Probe designed especially for genotyping assay?

I designed 2 dual hybridization probes for my single SNP. where I attach my fluorophore 5'-FAM-nnnn-BHQ1-3', and 5'-HEX-nnnnnn-BHQ2-3'. I need to run it on the MIC RT PCR machine by Biomolecular...

28 July 2024 5,658 0 View