Antibodies for Cerulean/CFP that do not cross-react with GFP?

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Loading of the same factor in more than one components, should i delete them?

I'm using the big five personality inventory in my questionnaire. In the factor analysis items of the Big Five appear to more than one component. I know that it is better to get rid of the items...

14 April 2020 9,790 3 View

Why did my mice die after stereotaxic surgery?

I performed yesterday 6 subretinal injections using Isofurane as an anesthetic. The surgery lasted something less than an hour, isoflurane was set to 2% and mice were treated with Metamizol and...

06 April 2018 284 3 View

Working antibody for staining glycinergic neurons in the mouse spinal cord?

Hi everyone! I have a question regarding staining glycinergic neurons in the spinal cord of Bl6 mice. The literature is kind of limited on this topic. I want to stain these neurons, I was thinking...

18 October 2016 530 3 View

Does anybody knows any article or book referring to art as therapy ?

both psychological or anthropological/social aspects

12 June 2016 4,703 23 View

Is anyone aware of any research on the microbiological monitoring of in situ monuments?

We are currently looking into some fossil samples coming from fossil trees exhibited in situ, and I have come across several microorganisms. Can anyone recommend any papers on monitoring and...

16 May 2014 4,410 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View