Dear All,

This question might be stupid to all who are expert in co transformation. I tried to co transform p-RARE and pQE80l containing my Gene of Interest into XL1 blue. I used 30 microgram/ml of Chloramphenicol and  100 microgram/ml of Ampicillin. Unfortunately, it did not work. Now I should try sequential transformation. But, my question is what is the recommended antibiotic concentrations of CAM and Amp for co-transformation. I tried 25 micrograms/ml of CAM previously as well. Do you think I should reduce the concentration of Amp to alleviate the selection pressure of Amp?