Is fluoro-jade staining possible with tissue-cleared brains?

I am hoping to stain whole mouse brain hemispheres with fluoro-jade C as a marker of degeneration. We are presently working to optimize this staining for tissue cleared with the LifeCanvas SHIELD...

10 March 2020 9,709 1 View

Does it help for a prospective graduate student to have a research proposal in hand when applying to grad school?

This coming semester, I will be teaching an undergraduate class on graduate school preparation for science majors. One of the main assignments during the class will be the assembly of a full...

17 December 2019 8,239 11 View

Looking for validated questionnaires to use with both children and parents on reporting Emotional Health/difficulties?

Hi there, I'm currently doing my MSc in Psychology of Education. I'm looking to answer the following research questions for my dissertation? a) Are there differences in self-reporting of...

13 December 2019 8,779 5 View

Can a cell membrane be overloaded with too many particles attempting to enter?

When more than one biomolecules attempt to enter into a cell membrane at once (in a large quantity) does it impede the transfection process?

23 July 2019 4,149 2 View

How to improve DNA yields using DNA extraction kits designed for soil or sediment samples?

02 November 2017 9,029 3 View

How do you respond to requests for article reprints for articles which were published in journal to which you assigned copyright?

If the publication journal 'owns' the article and the request is not for teaching/educational purposes from someone who is not a current collaborator, do such requests fall under 'fair use' or...

17 October 2017 5,325 3 View

Has anyone conducted research on child protection, self harm, sucide, etc in SE Asia, more specifically Laos if possible?

Looking for any research around child protection in Laos. GoL sensors everything so I'm looking for information that might not have been censored yet. Thank you!

16 August 2017 614 3 View

Can SPSS be used to carry out a Cox PH model with a time-varying exposure covariate that changes status more than once?

I have looked through some help documents that indicate that the time-varying covariate can be constructed for an individual that has been exposed and then unexposed, or vice-versa. I was...

26 July 2017 7,097 3 View

ANOVA versus Student's t-test_Small samples

Hi, I hesitate between two statistical tests (ANOVA and Student's t-test) to compare the means of three groups (unpaired, n

20 July 2017 6,168 6 View

Can stress/pain induce coactivation of the sympathetic and parasympathetic autonomic branches and if so; can it be measured with HRV quantification?

Relevant in humans and in animals.

12 May 2017 8,273 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Statistics: include groups of respondents made by one person for analysis?

Good morning, I could not find literature about this so I am asking. I am organizing data for a questionnaire (N=100) that foresees some personal questions like level of education. For the aim...

25 February 2021 2,090 4 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View