Grant January

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Questions related from Grant January

Antibacterial liquid inhibition assay (MIC) with LB medium?

Is it possible to determine the antibacterial minimum inhibitory concentration (MIC) using a liquid inhibition assay (MIC) with Lysogeny broth medium instead of Mueller-Hinton broth? If so, is...

10 February 2020 6,467 5 View

How to improve DNA yields using DNA extraction kits designed for soil or sediment samples?

I am trying to extract community DNA from deep-sea sediments using MP BIOMEDICALS FastDNA™ SPIN Kit for Soil. I have used starting material from 50 mg (recommended amount for marine sediments) to...

03 November 2017 9,113 3 View

Has anyone come across protocols for culturing marine bacteria from sea sediments under anoxic or microaerophilic conditions?

I am in search of protocols for culturing marine bacteria from sea sediments under anoxic or microaerophilic conditions. My samples are from deep-sea sediments 2-5 km in depth from Antarctic...

03 October 2017 9,364 6 View

Can anyone recommend a research article wherein crude extracted fucoidan was purified and an assessment of chemical composition was conducted?

Could someone please direct me to a research article where crude extracted fucoidan (or FCSP) was purified, and the chemical compositions between the crude and purified forms of fucoidan was...

19 August 2015 6,424 2 View

Why does eluting algal polysaccharides with a conc. gradient of EtOH give rise to fractions with varying molecular weights and chemical composition?

I am purifying fucose-containing-sulphated-polysaccharides/FCSP (potentially fucoidan) by anion exchange chromatography. I then complex fucoidan with CTAB creating a fucoidan-CTAB salt and run it...

11 August 2015 287 5 View

Are there commercially available fucoidanases or fucoidanase-like enzymes available for purchase?

I am looking for an enzyme that can hydrolyse a fucoidan polysaccharide into oligosaccharide units.

28 August 2014 273 1 View

Are there carbohydrate molecular markers available for the estimation of the molecular weight of carbohydrates on a C-PAGE?

I have isolated various marine polysaccharides (fucoidans) from several seaweeds and would like a rough estimation of their sizes (kDa) by running them on a carbohydrate polyacrylamide gel...

28 August 2014 3,057 7 View