I want to ask from researchers that if we made metallic nanoparticles with different methods then is it necessary that their morphology and biological action (i.e. antibacterial activity) will be different?
Probably yes in sense of different obtention method usally implies different morphology product, and in turn, morphology have influence over dynamic behaviour in solution and possible bacteria-nanoparticule interactions. However, is a very interesting research question.
Antibacterial properties depend on the synthesis of nanoparticles with antibacterial surfactants used in the synthesis or antibiotic. For example, our antibacterial surfactant s-alkyl isothiuronium halides. If nanoparticles are pure, their properties depend on the nature, specific surface and not synthetic methods.
Antimicrobial effect of metallic nanoparticles related to their properties which depend on their morphology not on synthesis method.
If you mean that synthesis method affect on morphology and therefore their properties including antimicrobial activity the answer will be "yes synthesis method and parameters affect indirectly on antimicrobial activity"
The antibacterial activity greatly depends on the size, surface charge and surface area of the nanoparticles. Now, if these properties of nanoparticles vary/ change with synthesis method then the antibacterial property will vary accordingly! It is well documented that particles with small size and higher surface area show enhanced antibacterial property. So, it is important to choose the synthesis method wisely.
The structure of the water does not depend on the method of preparation. The structure of the silver nanoparticles do not depend on the method of preparation. Morphology is not expressed by a number. Nanoparticle size (diameter) of numerically associated with a specific surface. Therefore, it is not necessary to say that the properties depend on the size and specific surface area. Antibacterial properties depend on the exact size of the nanoparticles, as well as all their properties.
They depend on the nature (structure) of nanoparticles and biological objects.