Our research group currently has some goat primary antibodies and we wish to use these to examine human skeletal muscle on western blot. Our current hrp conjugated rabbit anti goat secondary is giving us not specific binding at about 45kDa which is the same size as a protein we are interested in. So I was wondering has anybody found a good hrp conjugated anti goat secondary which they have used on human skeletal muscle samples and does not give not specific binding? We have tried a few different blocking steps and varying secondary antibody concentration but its not really suitable.
Perhaps the authors of the following publication can offer some meaningful suggestions (see section on "Western blot analysis and antibodies"):
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3707125/
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