Peter Durcan

9 Questions 19 Answers 0 Followers

Questions related from Peter Durcan

Phoenix ampho cell plastic adherence?

Hi I have recently thawed out a new vial from atcc of phoenix ampho cells and put them into a T75 flask but 24 hours later the majority of cells have not adhered and are in suspension. I was...

14 April 2017 8,556 2 View

How do I find out the specific nucleotide sequences used in the probe sets on microarrays for specific genes?

Searching through the GEO database and want to be able to know what was the sequences of the probes used to dectect gene expression but not sure how i can find out this specific information.

13 February 2015 8,307 2 View

Sequence is present in alternate assembly but not primary assembly what does this tell me?

I am currently looking at some splice variants of human genes and I have obtained sequence data from a PCR product which I had cut out of a gel. I have blasted this sequence against the human...

16 August 2013 8,952 1 View

Kinase binding to the extracellular domain of a transmembrane protein?

I am doing some work on type 1 transmembrane receptors and I have been using some bioinformatic tools to analyse possible protein domains present in them and one of the things that has come from...

29 July 2013 8,766 9 View

Anti goat secondary for human muscle western blot?

Our research group currently has some goat primary antibodies and we wish to use these to examine human skeletal muscle on western blot. Our current hrp conjugated rabbit anti goat secondary is...

22 July 2013 2,379 1 View

How would I go about sequencing a protein which is detected by antibody via western blot?

I'm currently investigating some proteins that have not been previously investigated in some human tissue samples and there is not much information available regarding these proteins in the...

19 April 2013 2,020 17 View

Cryosection problems with human skeletal muscle samples.

I have been doing some cryosectioning on frozen human skeletal muscle samples. The initial samples I worked with were not embedded in oct and were fine to cut on the cryostat. However, I now am...

14 March 2013 2,965 14 View

Quickest way to overexpress a gene

I would like to overexpress a gene in cell culture model. I have never done this before and from what i have read so far it typically involves establishing a stably expressing cell line usually...

26 January 2013 195 6 View

shRNA commercial supplier.

I am currently going through my options for knockdown and overexpression of genes in cell culture. I am interested in the shRNA option but have some questions as have no experience of using this...

26 January 2013 2,236 3 View