Hi!
I'm fine-tuning icv injection technique in mice, but I'm experiencing some problems with anesthesia. From what I've read, many authors use isoflurane. Still, I am interested in finding a long-lasting anesthetic (or a mixture) that can be administered ip, and that further allows the total recovery of the animal.
Could anyone shed new light on?
Thank you very much!
Fiona
Have you considered ketamine/xylazine? I have had good outcomes with survival surgeries with that combination administered IP.
Hi Kyle,
I know that it works great in rats, but never tried it in mice. Could you please help me with the doses?
Thanks
Fiona, Have a look at the attached document. I'm not sure what concentrations your stock solutions will be, but you can work backward from what is listed here.
Ketamine/Xylazine will definitely work in mice. However, if you are looking for a long-lasting anesthesia, be careful with only giving 2/3 of the final volume at first, and then you will have 1/3 to use to make it last longer. The only thing you don't want is an overdose.
Isoflurane is extremely stable and long lasting though, especially used with O2 instead of air.
Hi,
I join Marie's advices. We use isoflurane for ICV in mice and with O2 is really stable and long lasting and mice wake up quickly. Ketamine/Xylazine work good too but you can find more problems of overdose for a long-lasting effect (100mg/kg Ket/10mg/Kg Xyl).
Hope that helps!
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