hi, could someone tell me how to put these images from the immunofluorescence confocal? These images are from the pancreas and I marked the cells in green and red. I would like to overlay these images.
I find it more easy and straightforward in Photoshop where each channel can be overlayed as individual layers using the "screen" mixing option in the layer setting. Photoshop also allows you to quickly adjust the image histogram, crop the area of interest etc. Photoshop is not free, however.