After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

How to use -dhdl flag during mdrun with gromacs?

I am trying to use the gmx energy model to make free energy calculations upon binding of a ligand to a receptor. To do this I am invoking the energy module and adding the -odh flag: gmx energy -f...

09 November 2020 1,631 3 View

Preparation of mouse thymus for Flow Cytometry?

Hi, I'm trying to develop a protocol for prepping thymus tissues in mice. We will be using NSG mice in our lab and the goal is to get good consistent single cell suspensions to stain for FACS....

26 October 2020 10,006 3 View

Issues generating hbmap.xpm file from gromacs hbond module, using -xpm flag?

I am currently trying to monitor the changes that a ligand elicits in the hydrogen bonding pattern of a protein during binding. I can easily see with the -num flag that the number of internal...

18 August 2020 7,973 1 View

How to publish my old paper in an open journal?

Dear Research Gate Community, I'm finally able to publish my dissertation paper and I'm keen to find the right place to publish it. My old university has just given me permission to publish it...

13 July 2020 1,938 3 View

Recruitment of Children - Ages 5 - 12 year olds - best methods?

Hi All, I am running a study which requires two visits for children to complete an EEG (Brain scan) and an eye tracking task. We are currently working a recruitment plan for after the Covid-19...

30 March 2020 8,737 3 View

Which methods do academic writing and literacy facilitators apply to incorporate students' primary discourses into learning experiences?

Greetings Colleagues Primary discourses are those ways of using language, literacy and multiple modes of communicating as acquired in our homes, among our families and in our initial schooling...

28 December 2019 6,472 5 View

Is social media a good platform that will bring change in elections?

South African elections have ushered in a new thinking on the role of social media in influencing elections. The EFF had the highest number of young people who where on social media. ( 6 million)...

12 May 2019 4,031 6 View

How much solvent (ethanol) would I need to extract capsaicin (and other capsaicinoids) from siling haba?

Siling haba is a variation of Capsicum annuum var longum. The capsaicin extracted will be tested against aphids to test its insecticidal properties. Someone suggested that I should use 5ml/g or 2g...

12 February 2019 1,373 3 View

FMRI power analysis for RSA (representational similarity analysis)?

Dear community, I'm looking into ways how to do an a-priori power analysis for an fMRI experiment where the main analysis will be a representational similarity analysis (RSA). The experiment...

06 February 2019 1,338 2 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View