Hello everyone. I am trying to find alternatives to NK-92 cells for our ADCC assay. I know of KHYG-1 and the KILR1 CD16 line. Any others that have proven successful in your assays?
In my case, I want to measure the curvature of blue-marked particles in RGB color images, but the only possible way I've found to do it is by manually tracing the particles' perimeter and...
18 February 2024 583 7 View
The following call might be of interest for some of you here on ResearchGate. Would be happy to welcome you in September 2024 in Tbilisi at my university! CfP: Recovering & Uncovering the...
18 January 2024 8,606 0 View
Hi Everyone, I'm currently attempting to use Golgi-Cox staining to visualize dendritic arborization, but I'm struggling to get the full neuronal structure seen in classical Golgi-Cox staining. Has...
05 October 2023 4,251 0 View
Hi all, I am currently writing a paper on the possibility to predict song popularity on TikTok, based on audio features (e.g. danceability, valence, tempo). My dataset consists of the TikTok's...
01 July 2023 9,543 0 View
The isolated T cells will be further activated and expanded in vitro for transduction (with chimeric antigen receptors). The ex vivo culturing will take about 2-3 weeks. I know that for isolating...
11 June 2023 1,360 1 View
I found this tool by chance on the internet, but have not spoken to any user yet. Does anyone have experience with it? Is it possible to see one on site in Berlin? Many thanks, LG Oliver
13 April 2023 7,005 0 View
I have 3 groups: Intervention 1 (n=6) Intervention 2 (n=9) Treatment as usual (n=12) And I have 2 time points (pre and post intervention) What kind of test do you recommend? Thank you!
22 March 2023 4,245 3 View
Hello! am looking for a research question for my thesis for my bachelor's in business administration. I am really interested in Vertical Farming. Does anyone have any ideas?
23 January 2023 250 4 View
Recently we faced an issue where we transduced primary mouse T cells with CAR constructs + a fluorescent tag. We tracked the expression of the tag on day 0, 2, and 4 post-transduction and the tag...
07 January 2023 281 0 View
How to derive Equation 5.1.3 in High Impedance Electromagnetic Surface of Seivenpiper? The equation is as follows:
15 December 2022 4,871 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View