Gromacs 2020: how to make distance restrains for 2 atoms in different protein chains?

Sorry, I think it is rather simple problem to solve, but I am stuck on it. I have 2 protein chains, so I have 2 *.itp files for them that are loaded in topol.top. What should I do to use distance...

01 December 2020 1,496 1 View

Different Ct in qPCR with three sets of reference genes primers (each ref.gene is a single copy per genome), with the same template - is it normal?

I am trying to build up 3 standard curves for 3 sets of primers for different reference genes, in order to later use one with the best efficiency. (for SYBR green qPCR, gene copies number...

27 February 2018 8,313 3 View

QPCR with SYBR green - should the amplicons (GOI and reference) be the same length ?

Do I understand right: when running qPCR with SYBR green detection, it is important that two amplicons (GOI and reference gene) should be of the same lenght ? Once It is supposed to be relative...

07 February 2018 4,678 5 View

How do you assess the efficiency/ rate of mutagenesis, when you plate your culture on the selective plate (e.g. canavanine or 5-FOA) ?

After the chemical or UV mutagenesis, the yeast culture is supposed to be plated on the selective medium with canavanine or 5-FOA in order to assess the efficiency of mutagenesis. The questions...

04 January 2018 4,813 3 View

How to do non-water solvent parametrization for Molecular Dynamics?

Currently I need to parametrize some simple F and Cl containing solvents to use them in Gromacs. The problem is that common force fields do not contain atom-types I need, so while understanding...

18 October 2016 7,283 3 View

Could I expect any quantitative difference in EDX results, if the analysis is performed on SEM/TEM samples (same steel and area)?

INCA (Oxford Instruments), X-Max 80 Cr-Ni stainless steel

16 May 2016 7,436 9 View

How to solve the structure of a complex in crystallography?

22 February 2016 2,615 3 View

Could anyone help me with continuous casting modelling in Abaqus ?

11 October 2014 246 1 View

Diabetes data set for ANN

Hello. Can anyone help me with some useful data for my Master's diploma? I am making an Artificial Neural Network for prediction diabetes. I know about some open data sets like Pima Indians,...

01 January 1970 6,480 2 View

How to avoid fake journal articles published under your authorship without your permission?

01 January 1970 726 4 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View