Oleg Tyurin

4 Questions 6 Answers 0 Followers

Questions related from Oleg Tyurin

Alternative (other than glucose) feeding strategies for S. cerevisiae fed batch cultures. Can you recommend nice exhaustive reviews ?

I am searching for alternative (other that glucose) feeding strategies for S. cerevisiae fed batch cultures. Can you recommend nice exhaustive reviews or papers on that please ? I couldn't find...

07 August 2020 8,755 0 View

Different Ct in qPCR with three sets of reference genes primers (each ref.gene is a single copy per genome), with the same template - is it normal?

I am trying to build up 3 standard curves for 3 sets of primers for different reference genes, in order to later use one with the best efficiency. (for SYBR green qPCR, gene copies number...

28 February 2018 8,413 3 View

QPCR with SYBR green - should the amplicons (GOI and reference) be the same length ?

Do I understand right: when running qPCR with SYBR green detection, it is important that two amplicons (GOI and reference gene) should be of the same lenght ? Once It is supposed to be relative...

08 February 2018 4,803 5 View

How do you assess the efficiency/ rate of mutagenesis, when you plate your culture on the selective plate (e.g. canavanine or 5-FOA) ?

After the chemical or UV mutagenesis, the yeast culture is supposed to be plated on the selective medium with canavanine or 5-FOA in order to assess the efficiency of mutagenesis. The questions...

05 January 2018 4,922 3 View