How long can agarose embedded tissues be kept?

I am embedding PFA fixed tissues in low melting point agarose prior to sectioning and I am wondering how long can these can be kept whilst maintaining tissue integrity and fluorescent signal?...

14 February 2021 6,688 3 View

Can anyone direct me to a paper or chapter that discusses calculating sample size in a self-selecting survey study?

Our study is investigating clinical knowledge in a specific area in healthcare workers at a large metropolitan hospital. Participants will self-select to complete the survey via email invitation...

11 January 2021 7,934 2 View

Align list of short sequences to miRBase?

I have a list of short sequences from miRNA sequencing in an Excel format and want to align these to miRBase. How can I do this? Is there a function in R? I converted the Excel list into a fasta...

08 September 2020 9,908 3 View

How to get return loss below -10dB for monopole antenna which has very less or no ground plane (or directly interfaced with sma connector) ?

I am designing a monopole antenna For 59MHz frequency . The lamda length is 5m. I tried for lamda/2 which is directly interfaced to sma connector. Couldn't get proper return loss.( According to...

11 March 2020 4,885 2 View

Can anyone recommend a stain to identify mast cells/eosinophilic granular cells (EGC) in fish?

Hi, I was wondering if someone can recommend a stain where fish EGCs or mast cells show significant metachromasia. Toluidine blue did not work in my fish species. I did find recommendations on...

04 March 2020 7,817 1 View

Questions about RNA extraction?

Hello, I have a problem with my gene expression results. The results of my endogenous gene are oscillating between samples, generating false negative results. It takes about 12 hours after sample...

08 October 2019 569 3 View

Why did I get RMSD plot with 10ns if the MD was 100ns trajectory?

I am new in gromacs and I'm experiencing some difficulties in the analysis of my results. I calculated the RMSD of backbone and ligand from the MD with a trajectory of 100ns (nsteps = 50000000),...

04 September 2019 3,669 6 View

Doubt about secondary antibody to immunohistochemistry?

I bought a secondary antibody to use for immunohistochemistry, but the manufacturer says the antibody is only validated for Western Blotting, not Immuno. Because of this, I don't think which...

26 August 2019 5,356 7 View

High background on stain free blot after transfer and its complications for protein normalization(TGX Stainfree Biorad Gel)?

Hey, I have a question regarding the stain free blot technology of Biorad. After electroforesis I take a picture ("Stain-free gel") for activation of the trihalo-compound. Next I transfer the gel...

12 August 2019 7,734 8 View

What are the strongest and most relevant indicators that have lead patients to suffer from Muscle Tension Dysphonia? Are robust orators more at risk?

i am working on research on this topic. MT Dysphonia is my diagnosis. I am a teacher who is trying to overcome the short and long term effects of this silent disorder. I was first diagnosed in...

06 February 2019 4,656 1 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

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03 March 2021 5,360 2 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View