Is there recent literature on the local pollution impact of self driving cars?

I have a question about the local pollution impact of automated cars. The possible energy impacts are well understood, I think, even if the net effects are very uncertain: automated cars could...

02 September 2020 5,735 2 View

Where to find photo dataset of depressed people faces?

Hi, for a facial emotion recognition detection project, a friend asked me if I know where he could find a dataset with faces of depressed people. I found this deep learning facial emotion...

02 June 2020 1,623 1 View

Selective etchant of ZnS and Ge from graphite (C)?

Hello chemical friends, Does anybody knows a chemical etchant that could selectively etch away ZnS and Ge from graphite (C)? I couldn't find any simple answer to this question yet. Thanks for...

11 November 2019 1,516 2 View

Where can I find data on the use of fertilizers and pesticides in EU forestry ?

Hello, I'm looking for recent data on the use of fertilizers and pesticides in EU forestry. Would you know any EU website where I could find it ? Cheers, Laurent

31 October 2019 4,016 3 View

Is it necessary to add error bar on control condition on qPCR histogram that represent fold change ?

--Hi, a reviewer ask me to add error bar on my qPCR control condition. But this is what i think: Noteably, the error bar for the control group has a different statistical meaning as the error...

06 October 2019 7,289 3 View

Is that possible to write an article about one condition of a two conditions study?

To explain more in details. We undertook a cognitive experiment where participants performed a similar task (with different stimuli) in two conditions: one condition used as control condition and...

05 November 2018 7,732 2 View

What primers for barcoding on bacteria and protists ?

Hi, Do someone knows what primer(s) I could use for barcoding bacteria and protists ?

22 November 2017 3,555 2 View

Non-selective InGaAs/InP etch

Dear all, I am not chemist and I can't really find the answer to my question in the litterature. Could anybody suggest me a recipe for a non-selective wet etching of InGaAs and InP with similar...

18 May 2017 3,345 3 View

Steklov spectrum in an ellipse ?

Does anybody know whether the expression of Steklov spectrum (eigenvalues + eigenfunctions) for the 2d Laplace operator in an ellipse is available in the literature ? It seems to me that, as the...

22 October 2016 8,726 1 View

Could someone help me with my transformation issue?

I am writing to you regarding a problem I had with my sequencing results. Allow me to describe the situation for you.I cloned 79 pb of MPITS1 into plasmid TOPO10 and the colony-PCR results were...

01 June 2015 674 5 View

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02 March 2021 3,060 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

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28 February 2021 9,936 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Has anyone experienced changes in growth for cell culture when purchasing media from different companies?

I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines

24 February 2021 2,268 3 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View