-surf option on gromacs produces weird radial distribution?

Hey guys, the following command uses the -surf flag and it causes some unnormalized-looking g(r) (see attached png file): gmx_mpi rdf -s tpr.tpr -ref 'resname TST' -sel 'resname RER -f movie.xtc...

03 October 2020 4,025 2 View

Surface RDF in Gromacs?

Hello, I am trying to use the -surf option in gmx rdf in order to compute the rdf of certain molecules from the surface (nearest atoms) of my reference molecule . My script is: gmx rdf -s...

22 September 2020 5,097 10 View

COM pulling on gromacs?

Dear all, I am looking for a way to restrain the center of mass (COM) of two different molecules bathed in water, using GROMACS 2018. The only thing close I found on gromacs that does this is the...

07 September 2020 4,435 8 View

Is there a way to convert MPN (most probable number) to CFU (colony forming unit)?

I am conducting a meta-analysis and most of my studies report values for bacterial analysis in CFU. However, I have one study which reports its microbial values in MPN. What should I do?

29 April 2020 662 5 View

How to clone 3 pieces?

Hello, I have to clone 3 pieces together: 1. 2 Pcr made- the inserts. One 4200bp (13 ng/microliter), one 460bp(21ng/microliter) 2. Vector 3800bp(13ngng/mictoliter). All pieces were cut with...

27 April 2020 809 3 View

Subgroup analysis in small numbers allowed?

Hello, I had a question regarding subgroup analysis in small patient numbers. I am currently writing a paper on very rare tumours and the response to therapy in a real-world cohort, so no...

18 March 2020 4,983 16 View

Trying to produce lentivirus containing human-FasL in 293FT cells, but the cells keep dying after transfection. Anyone succeeded making FasL-Viruses?

I have a Lenti-FasL plasmid (CMV promoter) I bought from GeneCopoeia, and I am trying to produce Lentiviral particles containing the human FasL plasmid. For that I am using 293FT cells with...

24 July 2018 1,361 3 View

Since G6P dehydrogenase is a dimer, are 2 H+ ions released upon catalysis of G6P, not 1 as shown in the equation? Are they released as gas or ions?

I'm specifically interested in the reaction as it occurs in EMIT assays. Does H+ stay in solution, hence the need for precise buffering, or is it released as gas?

10 April 2018 4,123 6 View

Have you used the ProteinSimple Simple Western, and found that your molecular weights were different versus regular blot? How did you resolve this?

I am investigating the ProteinSimple Simple Western system, but have found that my proteins (and some ladders that I've used in the past) come out at very different molecular weights compared to...

10 November 2017 8,075 3 View

I am looking for a good resource for risk communication in public health. Can anyone make a recommendation?

05 June 2017 9,314 11 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View