I wish to evaluate insulin receptor density and affinity following different glucose and insulin concentrations. As several thyroid cancer cell lines are available in literature (human model), how would you base your screening and finally choose? I am not familiar with cell cultures, this is why I would start with cancer cells.
First, is the insulin receptor depending on the alternative splicing? If so, you have to check which variable exons are inserted and expressed in each cancer cell line.
Second, you have to investigate into the survival rate and proliferation change before and after the treatment with insulin and glucose in concentration-dependent manner. In other words, you have to check which cell lines are susceptible or refractory to the starvation.
The serum and medium (e.g. DMEM F12) is the same among those cell lines???
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