I'm looking for a culture medium that will allow me to differentiate cellulose-producing bacteria from the non producing ones.
I read something a while ago about an agar media that turned blue around cellulose producing colonies, but I'm not able to find it again.
We use Luria-Bertani plates lacking NaCl and supplemented with Calcofluor white(fluorescent brightener 28) at 50 micrograms/ml. Cellulose-producing colonies glow under uv light (366nm). ref: Romling et al, Molec Microbiol 28, 249-364 (2000).
Thank you very much Sarah.
I will try it
But I'm not able to find your reference... can you please verify it's correct?
Thank you again
ok, found it!! :-)
Multicellular and aggregative behaviour of Salmonella typhimurium strains is controlled by mutations in the agfD promoter
MOLECULAR MICROBIOLOGY
Volume 28, Issue 2, April 1998, Pages: 249–264, Ute Römling, Walter D. Sierralta, Kristina Eriksson and Staffan Normark
OOPS! I scrambled two papers. The 2000 paper, rather than the 1998 one you found, is the right reference: MOLECULAR MICROBIOLOGY Volume 36, pages 10-23, 2000. Ute Romling, M Rhode, A Olsen, S Normark, and J Reinkoster
Oops! I scrambled two papers. The 2000 one, rather than the 1998 one you found, is the right reference. MOLECULAR MICROBIOLOGY Volume 36, pages 10-23 (2000). Authors Ute Romling, M Rhode, A Olsen, S Normark, J Reinkoster
Hi Dr
Cellulolytic fungi can be isolated on a medium containing cellulose 1% instead of its original carbon source,
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