Maira P. Almeida

2 Questions 2 Answers 0 Followers

Questions related from Maira P. Almeida

Has anyone experienced high cell death during monoclonal cell line isolation after cells have been CRISPR-edited?

Hello! In order to isolate a reporter line, NPCs were electroporated with a CRISPR construct. The cells grow well as polyclonal, but when cultivating them as single cells for isolation of...

12 January 2022 2,098 4 View

After generating crRNA+tracrRNA heating at 95C, can it be maintained at -20C and be used in future experiments? Or crRNA+tracrRNA needs to be fresh?

I only have used sgRNAs. For the first time I used crRNA+tracrRNA and I kept what was left in the -20C. Can I use it again or is it better to prepare fresh crRNA+tracrRNA? Thank you :)

25 November 2021 1,587 0 View