Dear everyone,

We've been dealing with EndoCBH1 cells for a while now. And even using their specific recommended cell culture media from Human Cell Design (OptiBMax) they are very hard to proliferate and amplificate, taking a long time to obtain a considerable number of cells, which obviously increases their passages, which is undesired. And which also makes hard to amplificate + freeze vials.

Does anyone have any tips/advice that we might be missing? We have tried culturing them at different seeds (70.000-100.000/cm2), passaging them at different days (5-7) but nothing seems to make major differences. We coat them with ECM Sigma, fribonectin Sigma and DMEM 4500 mg/L.

We know everyone struggles with these cells, we'd just like to know if anyone has any relevant change to suggest that they saw it truly worked.

Thank you very much in advance to anyone willing to help

Bea