I was wondering if (to avoid fungal contamination during transformant selection) it is fine to add fungicide in the minimal media solution. Has anyone ever tried doing that?
I do not recommend this idea, because fungicides the minimal concentration, probablily should give mutations. Please trait to work with very good aseptic conditions.
Perea Dallos Margarita Thanks for your response, I would be obliged if you share the relevant paper where this has been reported (or are you speaking of your personal experience with fungicides causing mutations?)