Gel purification of linearized plasmid, gave low yield why?

I am using Qiagen Gel extraction kit for gel purification of linearized plasmid. The procedure is followed as per the kit. if i load 1ug DNA in agarose gel, i am getting 600ng DNA or less after...

16 October 2020 6,667 4 View

Dear researchers, In RE digestion with Nco I i am getting smearing. Can any one please advice?

well1- 2 log ladder well2- uncut plasmid(3.2kb) well3- cut wit NcoI well4-cut wit NcoI tried with less time of incubation, low amount of enzyme doesnot work

10 September 2020 6,711 3 View

How to find the list of journals with "Your Paper Your Way"?

03 May 2020 2,492 5 View

Request Articles /Research works related to Autonomous Spot weld quality prediction online (real time) using Machine learning techniques?

I am working on a similar project with few of our automotive supplier bases. It would help me to understand the prior experience if i read the related articles /researches / journals etc.. Thanks

15 September 2019 4,165 1 View

What does it mean when the slope of engineering stress strain curve decrease?

Engineering stress strain curve show a decreasing trend after yield point. How can we estimate the mechanical properties?

13 August 2018 9,824 1 View

Can anyone tell me how to get phi/psi angle vs time?

I would like to calculate the phi/psi angle vs. time for a particular residue in my protein. Can anyone tell me how to do this task?

01 April 2014 4,002 2 View

How to generate a hessian matrix file using Gromacs

I completed MD simulation with 25ns for my protein and i have to analyze normal mode analysis. For analyzing normal mode, i have to produce hessian matrix. please help me in this regard........

21 May 2013 9,939 2 View

What is the best method for Ponceau S preparation?

2g Ponceau-s, 30g TCA, 30g Sulfosalicylic acid or 0.1% Ponceau in 1% v/v acetic acid? How long should it take to visualize the bands?

19 July 2012 1,146 3 View

Singularity error in thermal stress analysis of WAAM (FEM simulation - COMSOL)

I have been trying to simulate an FEM model of wire arc additive manufacturing of just a layer of material on the substrate and trying to find the associated thermal stresses using COMSOL...

01 January 1970 4,267 2 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View