28 February 2019 3 9K Report
  • list would be good

    • More Palak Sehgal's questions See All
    Why does intensity drops as concentration increases?

    Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

    28 February 2021 3,653 4 View

    How to measure volume of Pinus roxburghii ?

    I want to measure volume of Pinus roxburghii in the Jammu region of India to calculate the carbon stocks of Pinus roxburghii.

    31 January 2021 2,311 3 View

    I'm facing some troubling in designing a anteena ....anyone tell me how give CPW feed in cst ?

    I want to give a CPW feed anyone suggest how to providea feed in antenna which I'm designed

    18 January 2021 3,517 2 View

    What is the need of having weights at the output layer of an RNN ?

    In RNN, Yt is the output which is given by the equation; Yt = Why.ht where Why is the weight associated with the output layer and ht is the current state. What is the need of having this weight...

    11 January 2021 8,710 4 View

    How to tackle the problem of constant val accuracy in CNN model training ?

    I am new to the field of Neural networks. Currently, I am working on training a CNN model to classify XRAY Images into Normal and Viral Pneumonia. I am facing an issue of Constant Val accuracy...

    29 October 2020 3,510 10 View

    How to check solubility of PHA?

    I have extracted mcl PHA from bacteria. But I am facing problem in dissolving the extracted PHA. It is not dissolving in chloroform and other solvents like acetone, dichloroethane, diethylether...

    29 October 2020 7,794 2 View

    Best metallurgy to prevent hot sulfidation?

    Please note that I am an inspection engineer and would like your views/ opinion on the following - We have experienced severe metal wastage and tube failure in a heat exchanger with SS304 tubes....

    26 March 2020 9,877 6 View

    How to dissolve Ethylene Vinyl Acetate?

    I need to get rid of the ethylene vinyl acetate paste stuck onto a glass surface. What would be the best solvent to get rid of it?

    09 August 2019 9,016 6 View

    Regarding property definition error in modeling reinforcement in reinforced concrete element?

    I have modeled the concrete as solid and reinforcement as truss element and I used embedded option to specify interaction between the reinforcement and concrete slab. I am sure that i have created...

    16 November 2017 2,637 3 View

    Why ZnS nanoparticles are suited as a photocatalyst?

    I made ZnS Quantum dots to use as a photocatalyst.

    15 June 2017 7,832 5 View

    Similar questions and discussions
    Buffer to disrupt platelet alpha granules for PF4 quantification?

    Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

    03 March 2021 1,499 2 View

    Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

    Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

    03 March 2021 6,299 3 View

    How optimize the Size Exclusion Chromatography?

    Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

    01 March 2021 2,215 2 View

    Adherance of Polyaniline coating over stainless steel?

    Is the electropolymerisation of polyaniline coating over stainless steel (SS) via Cyclic voltammetry technique is really adherant? Else how to improve the adhesion of the coating, whether any...

    01 March 2021 8,220 2 View

    Why are my bands weird in this Agarose gel?

    Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

    01 March 2021 9,952 3 View

    What do I do with DLS peaks that are from the buffer?

    I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

    01 March 2021 9,015 2 View

    How to classify strictly upper triangular matrices by similarity?

    Let F be a field. Consider U, the set of n times n strictly upper triangular matrices in F. For X, Y in U, we call them similar if there exists some S, which is non-degenerate and upper...

    01 March 2021 2,957 8 View

    Is there a method of determining feed digestibility without the need to re-pellet the feed?

    I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

    28 February 2021 7,967 3 View

    How to immerse bricks in wax ( for coating ) ?

    Do we immerse the bricks horizontally or vertically ? #bricks#waterproofingsurface#civil#engeenering#chemicalengineering#wax#parafinwax

    28 February 2021 6,685 2 View

    For gelatin coating for culturing HUVEC.Should the gelatin be made in sterile water or PBS? Do I need to autoclave the gelatin soln after making it ?

    I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?

    28 February 2021 274 2 View

    Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close