Dear Scientists
Is any database that allows me to the prediction of chromatin modifiers (like kdm6a, a histone demethylase / EZH2, a histone methyltransferase) regulate what transcriptional factors?
Thanks for your answers.
How could I get the P-value from the mean, SEM, and n of different groups in the t-test without the original data? For detail, I would like to know the P-value between BMP-2: 50ng/ml group and the...
06 February 2021 6,539 4 View
Hi guys I'm out of ideas here please help! I've never had consistent problem like this doing westerns before and I can't figure it out. The condition used: - First 4 lanes have 50ug protein in...
04 February 2021 2,297 3 View
I am searching for miRNA repeats put between WPRE and 3'LTR of my lentivirus, in order to enhance the specificity of transgene expression in human neuron cells. But if clinically translation of...
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Recently I started to use XRD to study the structure of some molecular sieves. My powder sample was prepared by grinding in a mortar, drying in an oven, and loading on the sample holder. However,...
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I have installed the software BRUKER Flexanalysis 3.4 on my computer successfully. But when I tried to import the MS data produced by BRUKER MALDI-TOF-MS, I met with an error described in the...
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Hi, As described, recently we found a gene was silenced in various tumor, and its promoter was alwasy hypermethlated. When using certain treatment to induce the gene expression, we found its...
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Hello, I'm working with immortalized HUVECs low and I'm trying to study their exosomal RNA contents. Could anyone recommend an effective kit to isolate exosomes from cell culture supernatant?...
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We need to collect CSF samples repeatedly from the 4th or lateral ventricle of rats. Does anyone know of a protocol for implanting a cannula to be able to do this? Or even a protocol for this...
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I am analyzing the tissue damage to the mouse cornea tissue after incubated a kind of medium. The protocol is 1) mouse cornea was incubated in the medium for one day 2) embedding the cornea with...
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Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...
01 March 2021 210 1 View
I have a dataset with 96 individuals and 1496 transcripts of interest. I have analyzed the experiment by multiway ANOVA in the Permanova platform of Primer-E. I am satisfied that I have figured...
13 February 2021 7,217 4 View
Hello, I would like to compare the expression levels of several genes in insects, so I prepared standard curves and estimated copy numbers of each sequence in my samples. But for one of the genes,...
11 February 2021 8,421 13 View
Hi all, I stained tdTomato+ cells (PBMCs and Jurkats) with eBioscience Foxp3 / Transcription Factor Staining Buffer Set for the foxp3 and I completely lost the signal of tdTomato. I tried to fix...
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I would like to evaluate the amount of reverse transcription (cDNA) on a 20um fixed mouse brain section. I am using random primers with RevertAid H Minus M-MuLV (reverse transcriptase). and would...
08 February 2021 1,957 4 View
I am working with a very unstable transcriptional factor. I use DNAse in the lysis buffer and I purify it as inclusion bodies, by using urea and 1M NaCl. After purification, I buffer exchange the...
05 February 2021 2,530 3 View
Hi guys. I make RNA by in vitro transcription and do cell transfection afterwards. I analyze RNA using Nanodrop and Bioanalyzer. But I found the concentration of RNA measured by these two machines...
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I am very new to quantitative PCR and need some help for this technique. For my experiment, I need to perform a quantitative reverse transcription PCR. I extract the RNA from my pellet and...
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Hi everyone, I would greatly appreciate it if someone can shed light on certain endothelial functional assays and their conditions. I often notice that protocols are run with serum-free media, or...
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I'm looking for a fixative that works quickly but you can safely store cells in for a longer period (i.e. a few hours). Most importantly it must be able to fix chromatin well and be compatible...
03 February 2021 7,659 7 View