Hi,
I recorded some abnormal EPSCs in my iPSC-derived neurons. Not like the individually separated EPSC signals, the ones appear in my Heterogeneous neurons which harbor the mutation associated with epilepsy, and these are some "Busting Like" EPSC events. It jumps to the maximum amplitude and stays there for quite some time and not going back (see attached pic). It only appears in my Het but not in control.
I am wondering how this happened, and any thoughts would be appreciated!
Thanks!
Instrumentation trouble. The offset level of electrical signal (from instrumentation amplifier) suffered deviation.
Thanks for your input Francisco Fambrini !
May I ask what is the setting I can try to fix it if the baseline suffered deviation?
However, the only happens when I am patching the iPSC-derived neurons (I am patching the 3D brain organoids)while I never came across it doing brain slices recording. I am wondering could it be some inherent difference between these two.
Thanks.
It's hard to answer without knowing your experiment deeply. I would need to check your entire experimental apparatus to give you some useful answer on this.
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