A way of bursts measures through opnet simulation ???

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A way of bursts measures through opnet simulation ???

I'm working in the research of the impact of bursts in the buffer and I don't know how I can measure the effects of this bursts in the buffer. I'm using Opnet simulation I want the way any one...

11 September 2017 5,030 2 View

Get cDNA sequences of a list of mRNAs?

Hi everyone I have a list of mRNA accession numbers. I'm trying to get cDNA sequence for all the mRNAs which are almost 3000. Is there a way to get all the sequences at once? Even the BioEdit has...

27 January 2017 655 3 View

Can someone help with the synthesis of silver nanoparticles?

I add 0.1 from crude cellulase to 9.9 ml silver nitrate and the solution is ok after 10 minutes: the color change. However after 2 h I found something in solution ( see the picture ) What could...

10 November 2015 8,338 4 View

AMAXA Lonza nucleofection lower volume of buffer?

Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?

07 August 2024 4,616 0 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

EDTA buffer solution?

Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.

29 July 2024 4,374 1 View

Whether i can preserve leaves samples in 2.5% glutaraldehyde in 0.05 M phosphate buffer, pH 7 for coming several weeks for further LM study ?

I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.

29 July 2024 8,560 0 View

Can a shoot-through event of a tri-state digital buffer cause momentary Hi-Z state?

// interested in the difference between floating events and short circuits.

22 July 2024 6,565 0 View

How can EDTA be removed from already extracted DNA samples?

Our lab has extracted bacterial DNA samples using Qiagen DNeasy kit and eluted the samples in AE buffer. However, this buffer contains 0.5mM EDTA not suitable for downstream application. Any ideas...

13 July 2024 1,675 3 View

How much 1X TAE buffer should I use in preparing 1.2% agarose gel?

Commonly, we prepare 1.0% of agarose gel with 0.4g agarose powder and 40ml 1X TAE buffer. if I would like to prepare a 1.2%% of agarose gel, is it I need to add 0.48g agarose powder and 120ml 1X...

12 July 2024 2,058 5 View

My Cyclic voltammetry curve doesn't show cathodic peak of Cu2+ to Cu+. how to solve this problem?

I performed CV of Cu-MOF in a buffer solution of pH 8, and we did not find any peak corresponding to Cu2+ to Cu+. What is the reason behind this? Please give your valuable suggestions.

08 July 2024 2,388 4 View

Shubham Gupta

Its simple you can set the burstiness of the traffic and then check the amount of packet processed/dropped in the buffer.

I dont know about OPNET but I can tell you how it can be done pretty quickly in NetSim.

Muhammad Asghar Khan

https://splash.riverbed.com/servlet/JiveServlet/previewBody/2306-102-1-2602/133.296.pdf