Hi community.
I was looking for a software or an R command to calculate the unbiased expected heterozygosity (uHe; Nei and Roychoudhury, 1974). Also, I would like to know if calculating the uHe will truly help me to avoid biases due different sample sizes in my studied populations, where the sample sizes of some populations are very small (4-5 individuals, while other populations have more than 15 individuals sampled)
Thanks a lot
Hi Ringo Urquia.
A very late reply, hopefully useful for others.
As far as I understand this, the unbiased estimator you want to use is that of Nei 1978 (Nei M 1978. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89: 583-590.), which is an update of the one proposed earlier by Nei and Roychoudhury, 1974. It is implemented by default in several R packages such as poppr (locus_table or poppr functions), or simply in the summary function in adegenet.
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