Hi everyone
As the question says, do we always have to exclude monomorphic loci from our analyses when using SSR markers?
Specially, I would like to know if this is true for insular populations for example, where we expect to find low diversity levels and maybe an only allele for some loci that are reported as polymorphic in broader populations such as continental ones.
Thanks all!
Dear Ringo
It is depended on your aim of study.
If you wanna estimate similarity among pop or genotypes, you should use both monomorphic and poly morphic bands (this is common in genetic diversity study).
Some time you wanna find just genetic diffrential, so you have to use polymorphic bands (this is common in genetic differntial studty).
Hi Mostafa
Thanks a lot for your answer. Precisely, we wish to compare the diversity among 3 insular populations. So it makes perfect sense to me considering monomorphic markers too as you said.
Thanks a lot.
Hi,
As Mostafa rightly said. It depends on the type of study you undertake.
Genetic differentiality or similarity can still be useful information for the genetic diversity per se.
Thanks
Thanks a lot Prahalada and Mostafa, what you said makes perfect sence to me.
I will consider your advices for our research.
Dear Ringo,
In my opinion, monomorphic in term of all individuals in the population are having homozygous genotypes (all show only one band) will not have any to the study that you do. SSR marker loci having single band in all evaluated individuals do not have added value in your marker analysis. You need polymorphic markers to have the added value.
You also need to have marker loci having the PIC (polymorphic information content) per locus values of at least 0.4 for diversity analysis. It is even more important in your case to select SSR marker loci having higher PIC value than 0.4.
Good luck in your researh...
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