Hi everyone!
How comparable are the expected heterozygosity (He) values obtained from the assessment of different kinds of molecular makers?
For example, if I have a He=0.5 from a study based on SSRs, and a He=0.1 from another research that used RAD markers, is it correct to conclude that the population studied with SSRs has a higher genetic diversity than the population studied with RAD?
Thank you for your help!
best,
DU
My opinion: absolutely not. Measures of heterozygosity are not directly comparable between different marker types due to their inherent properties. RAD (and SNPs in general) tend to have lower He than SSRs. It's just the product of the higher allelic diversity observed with SSRs.
Hope this helps.
My opinion: absolutely not. Measures of heterozygosity are not directly comparable between different marker types due to their inherent properties. RAD (and SNPs in general) tend to have lower He than SSRs. It's just the product of the higher allelic diversity observed with SSRs.
Hope this helps.
I’ll add, agree with Justin and you can only compare same method same locus as various sites will have different rates of He or Ho. If examining a pooled average be cautious of underlying bias. Also sample size can impact the H calc So keep that in mind as well.
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